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Interaction of proteins with aggregates of catecholamines and nucleotides: possible biological implications

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Abstract

Aggregates of noradrenaline and adenosine-5′-triphosphate (ATP) sediment jointly with gelatine on analytical ultracentrifugation. The sediumentation rate of this misture is higher than that of gelatine or of the aggregates alone. Furthermore, gelatine causes an increase in volume of the bottom phase which separates in aqueous solutions of ATP, noradrenaline and calcium. These effects are not obtained with the globular protein albumin.

It is concluded that gelatine reversibly binds noradrenaline-ATP aggregates. Since the chromogranins have similar physicochemical properties to gelatine, an interaction of these proteins with aggregates of ATP and noradrenaline may be anticipated to occur in the adrenal chromaffin granules.

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Authors and Affiliations

  1. Research Department, F. Hoffmann-La Roche & Co. Ltd., 4002, Basle, Switzerland

    K. H. Berneis, A. Pletscher & M. Da Prada

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  1. K. H. Berneis
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  2. A. Pletscher
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  3. M. Da Prada
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Berneis, K.H., Pletscher, A. & Da Prada, M. Interaction of proteins with aggregates of catecholamines and nucleotides: possible biological implications. Agents and Actions 2, 65–68 (1971). https://doi.org/10.1007/BF01965384

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  • DOI: https://doi.org/10.1007/BF01965384

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