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Assessment of the scavenging action of reduced glutathione, (+)-cyanidanol-3 and ethanol by the chemiluminescent response of the xanthine oxidase reaction

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Summary

The free radical scavenging capacity of reduced glutathione (GSH), (+)-cyanidanol-3 and ethanol was assessed by their interference with the maximal chemiluminescent response produced by the xanthine oxidase reaction. GSH and (+)-cyanidanol-3 induce a progressive inhibition of chemiluminescence when increasing amounts are added to the reaction mixture. GSH and (+)-cyanidanol-3 added together at low concentrations (1 and 0.05 mM respectively) exhibit an additive effect. The addition of enthanol presents a biphasic effect. It inhibits chemiluminescence at low concentrations (10–50 mM) while at higher concentrations (75–500 mM) this effect is reversed. Estimation of the concentrations required to produce half of the maximal inhibition of chemiluminescence by these agents revealed that ethanol is less effective than GSH and (+)-cyanidanol-3 as a free radical scavenger in the system used.

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Authors and Affiliations

  1. Unidad de Bioquímica, Departamento de Ciencias Biológicas, División de Ciencias Médicas Occidente, Facultad de Medicina, Universidad de Chile, Casilla 10455-Correo Central, Santiago, Chile

    L. A. Videla

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  1. L. A. Videla
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Additional information

These studies were supported by grant B-1162-822-3 from the Departamento de Desarrollo de la Investigación, Universidad de Chile. The author is grateful to Dr Alberto Boveris for valuable discussions and advice.

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Videla, L.A. Assessment of the scavenging action of reduced glutathione, (+)-cyanidanol-3 and ethanol by the chemiluminescent response of the xanthine oxidase reaction. Experientia 39, 500–502 (1983). https://doi.org/10.1007/BF01965175

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