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, Volume 12, Issue 5, pp 705–706 | Cite as

Radio-labelling of 1-O-alkyl, 2-O-acetyl,sn-glycero-3-phosphorylcholine, 1-O-(9,10-di3H)-octadecyl PAF-acether

  • J. L. Morgat
  • J. Roy
  • B. Wichrowski
  • E. Michel
  • F. Heymans
  • J. J. Godfroid
Platelets and Thrombosis

Abstract

3H-labelling of 1-3H-PAF-aceter was prepared by catalytic reduction of 1-O-(9,10)-octadecenyl-2-O-benzyl-sn-glyceryl-3-phosphorylcholine and then acetylated. 5 μmoles of this dehydro derivative dissolved in methanol was treated during 4 hours with tritium gas (80 Ci) and compressed until 1.1 bar by an automatic tritium transfer unit [1]. The catalyst was removed by filtration over Millex (Millipore) and labile tritium atoms eliminated by rotatory flash evaporation. The chemical purity was checked by3H scanning and autoradiography. It was finally recovered through preparative thin-layer chromatography and then acetylated. The specific radioactivity was found to be close to 40–50 Ci/mmole.

Keywords

Methanol Tritium Dehydro Octadecyl Specific Radioactivity 

References

  1. [1]
    F. Heymans, E. Michel, M.C. Borrel, B. Wichrowski, J.J. Godfroid, O. Convert, E. Coeffier, M. Tence andJ. Benveniste,New total syntheis and high resolution 1 H NMR spectrum of platelet-activating factor, its enantiomer and racemic mixtures, Biochim. biophys. Acta666, 230–237 (1981).PubMedGoogle Scholar
  2. [2]
    J.L. Morgat, J. Desmares andM. Cornu,Dispositif automatique de transfert de gas (tritium, deuterium et hydrogene), Jnl Labelled Compounds11, 257–264 (1975).Google Scholar

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • J. L. Morgat
    • 1
  • J. Roy
    • 1
  • B. Wichrowski
    • 2
  • E. Michel
    • 2
  • F. Heymans
    • 2
  • J. J. Godfroid
    • 2
  1. 1.Service de Biochimie, Departement de BiologieCEA-SaclayGif sur Yvette
  2. 2.Laboratoire de Pharmacochimie MoleculaireUniversité de Paris VIIParis Cedex 05France

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