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Radio-labelling of 1-O-alkyl, 2-O-acetyl,sn-glycero-3-phosphorylcholine, 1-O-(9,10-di3H)-octadecyl PAF-acether

  • Platelets and Thrombosis
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Abstract

3H-labelling of 1-3H-PAF-aceter was prepared by catalytic reduction of 1-O-(9,10)-octadecenyl-2-O-benzyl-sn-glyceryl-3-phosphorylcholine and then acetylated. 5 μmoles of this dehydro derivative dissolved in methanol was treated during 4 hours with tritium gas (80 Ci) and compressed until 1.1 bar by an automatic tritium transfer unit [1]. The catalyst was removed by filtration over Millex (Millipore) and labile tritium atoms eliminated by rotatory flash evaporation. The chemical purity was checked by3H scanning and autoradiography. It was finally recovered through preparative thin-layer chromatography and then acetylated. The specific radioactivity was found to be close to 40–50 Ci/mmole.

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References

  1. F. Heymans, E. Michel, M.C. Borrel, B. Wichrowski, J.J. Godfroid, O. Convert, E. Coeffier, M. Tence andJ. Benveniste,New total syntheis and high resolution 1 H NMR spectrum of platelet-activating factor, its enantiomer and racemic mixtures, Biochim. biophys. Acta666, 230–237 (1981).

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Authors and Affiliations

  1. Service de Biochimie, Departement de Biologie, CEA-Saclay, 91190, Gif sur Yvette

    J. L. Morgat & J. Roy

  2. Laboratoire de Pharmacochimie Moleculaire, Université de Paris VII, 2, Place Jussieu, F-75251, Paris Cedex 05, France

    B. Wichrowski, E. Michel, F. Heymans & J. J. Godfroid

Authors
  1. J. L. Morgat
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  2. J. Roy
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  3. B. Wichrowski
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  4. E. Michel
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  5. F. Heymans
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  6. J. J. Godfroid
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Morgat, J.L., Roy, J., Wichrowski, B. et al. Radio-labelling of 1-O-alkyl, 2-O-acetyl,sn-glycero-3-phosphorylcholine, 1-O-(9,10-di3H)-octadecyl PAF-acether. Agents and Actions 12, 705–706 (1982). https://doi.org/10.1007/BF01965085

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  • DOI: https://doi.org/10.1007/BF01965085

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