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Measurement of tele-methylhistamine and histamine in human cerebrospinal fluid, urine, and plasma

Agents and Actions volume 12, pages 583–590 (1982)Cite this article

Abstract

A gas chromatographic-mass spectrometric method described by us to measure tele-methylhistamine (t-MH) in brain was used to measure t-MH in human cerebrospinal fluid (CSF), urine and plasma. The presence of t-MH in these body fluids was rigorously established. No pros-methylhistamine could be detected, and it was used as internal standard to quantify t-MH in the fluids. The mean levels of t-MH were: urine, 943 pmol/mg creatinine; plasma, 12.3 pmol/ml; and CSF, 2.2 pmol/ml. Parallel measurements of histamine by a radioenzymatic method showed, respectively, 182 pmol/mg creatinine; 19.5 pmol/ml; and 388 pmol/ml. The levels of HA in CSF, much higher than those of its metabolite, t-MH, are high enough to stimulate HA receptors in the central nervous system.

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  1. Department of Pharmacology, Mount Sinai School of Medicine of the City University of New York, One Gustave L. Levy Place, 10029, New York, New York, USA

    J. K. Khandelwal, L. B. Hough, A. M. Morrishow & J. P. Green

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  1. J. K. Khandelwal
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Khandelwal, J.K., Hough, L.B., Morrishow, A.M. et al. Measurement of tele-methylhistamine and histamine in human cerebrospinal fluid, urine, and plasma. Agents and Actions 12, 583–590 (1982). https://doi.org/10.1007/BF01965064

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Keywords

  • Nervous System
  • Central Nervous System
  • Creatinine
  • Histamine
  • Cerebrospinal Fluid