Utilization of phosphate compounds for growth ofTetrahymena
Taking advantage of a synthetic nutrient medium, we have studied which compounds phosphate-starvedTetrahymena thermophila can use as phosphate sources for growth and cell multiplication. Ortho-, trimeta- and α-glycero-phosphate are good sources for both the wild type and a food-vacuoleless mutant; phosphorylcholine is used only by the wild type, and 2-aminoethyl phosphonic acid fails to serve as a phosphate source. Since at least two phosphatases are found in the extracellular fluid these results indicate thatTetrahymena can make use of extracellular digestion of nutrients.
Key wordsTetrahymena growth studies utilization of organic phosphate role of phagocytosis
- 4.Silberstein, G.B., J. Protozool.26 (1973) 519.Google Scholar
- 5.Kandatsu, M., and Horiguchi, M., Agr. Biol. Chem.26 (1962) 721.Google Scholar
- 6.Roberts, E., Simonsen, D.G., Kandatsu, M., and Kittredge, J., Science159 (1968) 886.Google Scholar
- 7.Thompson, G.A. Jr, in: New Comprehensive Biochemistry, vol. 4, p. 95. Eds T. Hori and Y. Nozawa. 1982.Google Scholar