, Volume 41, Issue 12, pp 1593–1594 | Cite as

Utilization of phosphate compounds for growth ofTetrahymena

  • L. Rasmussen
  • H. Toftlund
  • M. Florin-Christensen
  • J. Florin-Christensen
Short Communications


Taking advantage of a synthetic nutrient medium, we have studied which compounds phosphate-starvedTetrahymena thermophila can use as phosphate sources for growth and cell multiplication. Ortho-, trimeta- and α-glycero-phosphate are good sources for both the wild type and a food-vacuoleless mutant; phosphorylcholine is used only by the wild type, and 2-aminoethyl phosphonic acid fails to serve as a phosphate source. Since at least two phosphatases are found in the extracellular fluid these results indicate thatTetrahymena can make use of extracellular digestion of nutrients.

Key words

Tetrahymena growth studies utilization of organic phosphate role of phagocytosis 


  1. 1.
    Rasmussen, L., and Modeweg-Hansen, L., J. Cell Sci.12 (1973) 275.PubMedGoogle Scholar
  2. 2.
    Orias, E., and Pollock, N.A., Exp. Cell Res.90 (1975) 345.PubMedGoogle Scholar
  3. 3.
    Müller, M., J. Cell Biol.52 (1972) 478.PubMedGoogle Scholar
  4. 4.
    Silberstein, G.B., J. Protozool.26 (1973) 519.Google Scholar
  5. 5.
    Kandatsu, M., and Horiguchi, M., Agr. Biol. Chem.26 (1962) 721.Google Scholar
  6. 6.
    Roberts, E., Simonsen, D.G., Kandatsu, M., and Kittredge, J., Science159 (1968) 886.Google Scholar
  7. 7.
    Thompson, G.A. Jr, in: New Comprehensive Biochemistry, vol. 4, p. 95. Eds T. Hori and Y. Nozawa. 1982.Google Scholar
  8. 8.
    Rasmussen, L., Toftlund, H., and Suhr-Jessen, P., J. Cell Physiol.122 (1985) 155.PubMedGoogle Scholar
  9. 9.
    Suhr-Jessen, P., and Rasmussen, L., Exp. Cell Res.139 (1982) 457.PubMedGoogle Scholar
  10. 10.
    Orias, E., and Rasmussen, L., J. Cell Sci.36 (1979) 343.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • L. Rasmussen
    • 1
  • H. Toftlund
    • 1
  • M. Florin-Christensen
    • 1
  • J. Florin-Christensen
    • 1
  1. 1.Institute of Anatomy and Cytology, and Department of ChemistryOdense UniversityOdenseDenmark

Personalised recommendations