Storage of irradiated human blood; a source of error in quantitative chromosome analysis

Summary

Human whole blood was irradiated with 2.5 Gy of 220 kVp X-rays and stored before culture with 9.7 μM BrdU and 19.4 or 38.7 μM BrdU for 0, 24, 48 and 72 h. The frequency of dicentrics and ring chromosomes was determined in cells staining as first division (M1) metaphases with the fluorescence plus Giemsa technique. Storage had no influence on the observed aberration yields in 44 h cultures containing 9.7 μM BrdU. In 66 h cultures at 19.4 μM BrdU the observed yields after 2 and 3 days' storage were significantly lower as compared to cultures from fresh blood. No storage effect was revealed in 66 h cultures containing 38.7 μM BrdU. In cases where cytogenetic radiation dosimetry has to be carried out using blood samples which have been in transit for 2–3 days, the findings are of relevance for a correct determination of the chromosome damage in M1 cells.