Summary
A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue.
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Steinsvåg, S.K., Laerum, O.D. Fetal rat brain hemisphere tissue in nonadherent stationary organ culture. Experientia 41, 1517–1524 (1985). https://doi.org/10.1007/BF01964788
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DOI: https://doi.org/10.1007/BF01964788