Phenotype analysis of “inflammatory” macrophages in rheumatoid arthritis by two-colour cytofluorometry and immunohistology
Several monocyte/macrophage surface antigens (CD13, MHC II, Ki-M8) were found to be overexpressed in various compartments i.e. peripheral blood, synovial fluid and inflamed synovial membrane of RA patients. A decreased expression of CD11a (LFA-1) on CD14+ cells could be observed in RA synovial membranes and in synovial fluid as compared to PMNC. This finding was confirmed by immunohistology. Synovial lining cells lacked CD11a, but expressed CD11b and c. Although speculative, one could imagine that a CD14+ subpopulation of macrophages expressing CD11a in the circulation either does not migrate to the lining layer or loses CD11a during migration and differentiation.
KeywordsRheumatoid Arthritis Arthritis Synovial Fluid Surface Antigen Phenotype Analysis
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