Skip to main content
SpringerLink
Log in

Induction of superoxide production in rabbit neutrophils by a stable analogue of GTP

  • Endothelium — Cell Interactions
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Treatment of rabbit neutrophils with the stable GTP analogue GTPγS results in generation of superoxide. Whereas poly-l-arginine, an agent known to damage the plasma membrane, has only a small effect by itself, a strong and synergistic enhancement of superoxide generation is obtained in the presence of GTPγS and poly-l-arginine. The effect is potentiated in the presence of NADPH. Other nucleotides, such as GTP, are not effective in inducing superoxide generation. The results indicate that the stable guanine nucleotide GTPγS may activate superoxide production in rabbit neutrophils, and that this effect is evident if the plasma membrane has been permeabilized by poly-l-arginine, allowing an easy entry of GTPγS into the cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. M. Omann, R. A. Allen, G. m. Bokoch, R. G. Painter, A. E. Traynor, and L. A. Sklar,Signal transduction and cytoskeletal activation in the neutrophil. Physiol. Rev.67, 285–322 (1987).

    PubMed  Google Scholar 

  2. J. E. Smolen and S. J. Stoehr,Guanine nucleotides reduce the free calcium requirement for secretion of granule constituents from permeabilized human neutrophils. Biochim. Biophys. Acta889, 171–178 (1986).

    PubMed  Google Scholar 

  3. J. Doussiere, M. C. Pilloud, and P. V. Vignais,Activation of bovine neutrophil oxidase in a cell free system. GTP-dependent formation of a complex between a cytosolic factor and a membrane protein. Biochem. Biophys. Res. Commun.152, 993–1001 (1988).

    PubMed  Google Scholar 

  4. J. G. R. Elferink and M. Deierkauf,Permeabilization and calcium-dependent activation of rabbit polymorphonuclear leukocytes by poly-l-arginine. Inflammation,13, 285–294 (1989).

    PubMed  Google Scholar 

  5. P. E. Nasmith, G. B. Mills and S. Grinstein,Guanine nucleotides induce tyrosine phosphorylation and activation of the respiratory burst in neutrophils. Biochem. J.257, 893–897 (1989).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Biochemistry, University of Leiden, POB 9503, 2300 RA, Leiden, The Netherlands

    J. G. R. Elferink, G. J. J. C. Boonen, B. M. de Koster & M. Deierkauf

Authors
  1. J. G. R. Elferink
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. J. J. C. Boonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. M. de Koster
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Deierkauf
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elferink, J.G.R., Boonen, G.J.J.C., de Koster, B.M. et al. Induction of superoxide production in rabbit neutrophils by a stable analogue of GTP. Agents and Actions 29, 32–34 (1990). https://doi.org/10.1007/BF01964712

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01964712

Keywords

Search

Navigation