Abstract
Stuart's transport medium with a charcoal impregnated swab was tested for transport of biopsies from the gastric antrum for culture ofHelicobacter pylori. Biopsies were cultured under microaerophilic conditions either within 2 h or after a delay of 24 h at 4 °C. In 65 patients referred for gastroscopy two biopsies were taken.Helicobacter pylori was found in 39 patients. The rate of survival ofHelicobacter pylori was found to be as high in Stuart's transport medium after 24 h at 4 °C as in the paired biopsy which was cultured immediately after transportation in normal saline. In five (13 %) of 39 patients positive forHelicobacter pylori, the organism was cultured from only one of the biopsies. It is therefore recommended that two biopsies be taken for culture.
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References
Marshall BJ, Warren JR Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984, i: 1311–1315.
Buck GE Campylobacter pylori and gastroduodenal disease. Clinical Microbiology Reviews 1990, 3: 1–12.
Goodwin CS, Blincow ED, Warren JR, Waters TE, Sanderson CR, Easton L Evaluation of cultural techniques for isolatingCampylobacter pylori from endoscopic biopsies of gastric mucosa. Journal of Clinical Pathology 1985, 38: 1127–1131.
Westblom TU, Madan E, Kemp J, Subik MA, Tseng J: Improved isolation ofCampylobacter pylori from gastric biopsies using a biphasic transport media. Gastroenterology 1988, 94: A494.
Coghlan JG, Gilligan D, Humphreys H, McKenna D, Dooley C, Sweeney E, Keane C, O'Morain C Campylobacter pylori and recurrence of duodenal ulcers — a twelve month follow-up study. Lancet 1987, ii: 1109–1111.
Hartmann D, von Graevenitz A A note on name, viability and urease tests ofCampylobacter pylori. European Journal of Clinical Microbiology 1987, 6: 82–83.
Stuart RD, Toshach SR, Patsula TM The problem of transport of specimens for culture of gonococci. Canadian Journal of Public Health 1954, 45: 73–83.
Andersen LP, Holck S, Povlsen CO, Elsborg L, Justesen T Campylobacter pyloridis in peptic ulcer disease. Scandinavian Journal of Gastroenterology 1987, 22: 219–224.
McNulty CAM, Dent JC, Curry A, Uff JS, Ford GA, Gear MWL, Wilkinson SP New spiral bacterium in gastric mucosa. Journal of Clinical Pathology 1989, 42: 585–591.
Archer JR, Romero S, Ritchie AE, Hamacher ME, Steiner BM, Bryner JH, Schell RF Characterization of an unclassified microaerophilic bacterium associated with gastritis. Journal of Clinical Microbiology 1988, 26: 101–105.
Hoffmann S, Jensen AM, Justesen T The recovery of anaerobic bacteria from swabs in three transport systems. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1983, 91: 23–26.
Justesen T, Jensen AM, Hoffmann S The survival of anaerobic bacteria at 4 °C and 22 °C on swab in three transport systems. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1983, 91: 17–22.
Faoagali JL, Troughton D, Aitken J, Gwynne JF Campylobacter-like organisms in gastric biopsies: a Christ-church study. New Zealand Medical Journal 1986, 99: 50–52.
Karim QN, Maxwell RH Survival ofCampylobacter pylori in artificially contaminated milk. Journal of Clinical Pathology 1989, 42: 778.
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Kjøller, M., Fischer, A. & Justesen, T. Transport conditions and number of biopsies necessary for culture ofHelicobacter pylori . Eur. J. Clin. Microbiol. Infect. Dis. 10, 166–167 (1991). https://doi.org/10.1007/BF01964450
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DOI: https://doi.org/10.1007/BF01964450