Summary
The presence of cytoplasmic contaminants is demonstrated in preparations of rat liver nuclei, even after washing with Triton X-100. They can be removed by incubation at 37°C in a medium of low-salt concentration.
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C. Viticchi and F. Szeszák, Z. Alternsforsch., in press (1979).
F. Szeszák, Acta biochim. biophys. Acad. Sci. Hung.11, 113 (1976).
G. Blobel and V.R. Potter, Science154, 1662 (1966).
A.J. MacGillivray, A. Cameron, R.J. Krauze, D. Rickwood and J. Paul, Biochim. biophys. Acta277, 384 (1972).
U.K. Laemmli, Nature227, 680 (1970).
J.S. Bhorjee and T. Pederson, Analyt. Biochem.71, 393 (1976).
W. Schaffner and C. Weissmann, Analyt. Biochem.56, 502 (1973).
K. Burton, Biochem. J.62, 315 (1956).
W.C. Schneider, in: Methods in Enzymology, vol.III, p. 681. Academic Press, New York 1957.
J.B. Martin and P.M. Doty, Analyt. Chem.21, 965 (1949).
J.R. Tata, M.J. Hamilton and R.D. Cole, J. molec. Biol.67, 231 (1972).
D.B. Carter and C.B. Chae, Biochemistry15, 180 (1976).
F. Szeszák and L.A. Pinna, in preparation.
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The helpful comment of Prof. J.R. Tata in the preparation of the manuscript is kindly acknowledged.
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Viticchi, C., Szeszák, F. Cytoplasmic contaminants in Triton X-100 washed rat liver nuclei — a possible way of further purification. Experientia 35, 328–330 (1979). https://doi.org/10.1007/BF01964331
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DOI: https://doi.org/10.1007/BF01964331