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Regeneration of plants from mesophyll protoplasts ofAtropa belladonna

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Summary

Isolated leaf mesophyll protoplasts ofAtropa belladonna when cultured in defined liquid culture media regenerate cell walls, divide and form calli. Subsequent induction of shoot and root organogenesis leads to plantlets which grow to maturity after transfer to soil.

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References

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  2. F. D'Amato, Proc. 4th int. Congr. Pl. Tissue Cell Culture, Calgary 1978 (in press).

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  4. Culture media abbreviations: AE S. Arnold and T. Eriksson, Physiologia Pl.39, 257 (1977); DPD J. Durand, I. Potrykus and G. Donn, Z. Pflanzenphysiol.69, 26 (1973); KM K.N. Kao and M.R. Michayluk, Planta126, 105 (1975); MS T. Murashige and F. Skoog, Physiologia Pl.15, 473 (1972); NN J.P. Nitsch and C. Nitsch, Science163, 85 (1969); NT T. Nagata and I. Takebe, Planta99, 12 (1971); V47 H. Binding, Z. Pflanzenphysiol.74, 327 (1974).

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  5. Phytohormone abbreviations: BAP 6-benzylaminopurine; CPA p-chlorophenoxy acetic acid; IAA indole acetic acid; KIN 6-furfurylaminopurine.

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Authors and Affiliations

  1. Friedrich Miescher-Institut, P.O. Box 273, CH-4002, Basel, Switzerland

    H. Lörz & I. Potrykus

Authors
  1. H. Lörz
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  2. I. Potrykus
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Additional information

Acknowledgment. We are indebted to Václav Mandák for skilful technical assistance.

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Lörz, H., Potrykus, I. Regeneration of plants from mesophyll protoplasts ofAtropa belladonna. Experientia 35, 313–314 (1979). https://doi.org/10.1007/BF01964320

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  • DOI: https://doi.org/10.1007/BF01964320

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