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Resistance to ethidium bromide inAspergillus nidulans

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Summary

A mutant ofAspergillus nidulans resistant to ethidium bromide was isolated and the semi-dominant gene responsible for this resistance was allocated on linkage group II at 17.42±3.05 units of recombination from thewA3 gene. The gene also confers cross-resistance to acriflavin, malachite green and crystal violet. It was also shown that riboflavin is antagonistic to the toxic effect of ethidium bromide, at certain concentrations. The mechanisms which could be responsible for the toxic effect of this drug are discussed and compared with those of acriflavin. The use of theEtb 1 gene in genetical analysis through the parasexual cycle is suggested.

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Authors and Affiliations

  1. Department of Genetics and Evolution, Institute of Biology, University of Campinas, 13100 Campinas, São Paulo, Brasil

    M. E. Scarazzatti, R. Bonatelli Jr. & J. L. Azevedo

Authors
  1. M. E. Scarazzatti
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  2. R. Bonatelli Jr.
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  3. J. L. Azevedo
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Acknowledgements. The authors are thankful to the National Council for the Development of Science and Technology (CNPq) for financial support through PIG-SIP 04/053 FAPESP.

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Scarazzatti, M.E., Bonatelli, R. & Azevedo, J.L. Resistance to ethidium bromide inAspergillus nidulans. Experientia 35, 307–308 (1979). https://doi.org/10.1007/BF01964316

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