Skip to main content

Advertisement

SpringerLink
Log in

Identification ofCorynebacterium jeikeium andCorynebacterium CDC group D 2 with the API 20 strep system

  • Notes
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

A total of 170 strains ofCorynebacterium jeikeium and 23 strains ofCorynebacterium group D2 were examined in three British laboratories using the API 20 Strep identification system and three supplementary tests (catalase production, urease production and nitrate reduction). The isolates were collected from clinical specimens in various laboratories over a three-year period. The two species produced consistent reactions in these tests after 24 h. Two tests were highly discriminatory, with positive reactions for ribose fermentation seen forCorynebacterium jeikeium while urease production was observed withCorynebacterium group D2. This method allows routine clinical laboratories to rapidly identify these emerging pathogens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jackman, P. J. H., Pitcher, D. G., Pelcynska, S., Borman, P. Classification of corynebacteria associated with endocarditis (group JK) asCorynebacterium jeikeium sp. nov. Systematic and Applied Microbiology 1987, 9: 83–90.

    Google Scholar 

  2. Riley, P. S., Hollis, D. G., Utter, G. B., Weaver, R. E., Baker, C. N. Characterisation and identification of 95 diphtheroid (group JK) cultures isolated from clinical specimens. Journal of Clinical Microbiology 1979, 9: 418–424.

    PubMed  Google Scholar 

  3. Johnson, W. D., Kaye, D. Serious infections caused by diphtheroids. Annals of the New York Academy of Science 1976, 174: 568–576.

    Google Scholar 

  4. Hande, K. R., Witebsky, F. G., Brown, M. S., Schulman, C. B., Anderson, S. E., Levine, A. S., MacLowry, J. D., Chabner, B. A. Sepsis with a new species ofCorynebacterium. Annals of Internal Medicine 1976, 85: 423–426.

    PubMed  Google Scholar 

  5. Hoffman, S., Ersgaard, H., Justesen, T., Friis, H. Fatal meningitis with group JKCorynebacterium in a leucopenic patient. European Journal of Clinical Microbiology 1983, 2: 213–215.

    PubMed  Google Scholar 

  6. Pierard, D., Lauwers, S., Mouton, M. C., Sennesael, J., Verbeden, D. Group JKCorynebacterium peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. Journal of Clinical Microbiology 1983, 18: 1011–1014.

    PubMed  Google Scholar 

  7. Fernandez-Roblas, R., Prieto, S., Santamaria, M., Ponte, C., Soriano, F. Activity of nine antimicrobial agents againstCorynebacterium group D2 strains isolated from clinical specimens and skin. Antimicrobial Agents and Chemotherapy 1987, 31: 821–822.

    PubMed  Google Scholar 

  8. Soriano, F., Ponte, C., Santamaria, M., Aguado, J. M., Wilhelmi, I., Vela, R., Delatte, L. C. Corynebacterium group D2 as a cause of alkaline encrusted cystitis: report of four cases and characterisations of the organisms. Journal of Clinical Microbiology 1985, 21: 788–792.

    PubMed  Google Scholar 

  9. Aguado, J. M., Ponte, C., Soriano, F. Bacteriuria with a multiply resistant species ofCorynebacterium (Corynebacterium group D2): an unnoticed cause of urinary tract infection. Journal of Infectious Diseases 1987; 156: 144–150.

    PubMed  Google Scholar 

  10. Marshall, R. J., Routh, K. R., MacGowan, A. P. Corynebacterium CDC group D2 bacteraemia. Journal of Clinical Pathology 1987, 40: 814–814.

    PubMed  Google Scholar 

  11. Cowan, S. T., Steel, K. J. Manual for identification of medical bacteria. Cambridge University Press, Cambridge, 1975, p. 171–180.

    Google Scholar 

  12. Kelly, M. C., Smith, I. D., Anstey, R. J., Thornley, J. H., Rennie, R. P. Rapid identification of antibiotic resistant corynebacteria with the API 20S system. Journal of Clinical Microbiology 1984, 19: 245–247.

    PubMed  Google Scholar 

  13. Slifkin, M., Gil, G. M., Engwall, C. Rapid identification of group JK and other corynebacteria with the Minitek system. Journal of Clinical Microbiology 1986, 24: 177–180.

    PubMed  Google Scholar 

  14. Grasmick, A. E., Bruckner, D. A. Comparison of Rapid Identification Method and conventional substrates for identification ofCorynebacterium group JK isolates. Journal of Clinical Microbiology 1987, 25: 1111–1112.

    PubMed  Google Scholar 

  15. Bayston, R., Higgins, J. Biochemical and cultural characteristics of “JK” coryneforms. Journal of Clinical Pathology 1986, 39:654–660.

    PubMed  Google Scholar 

  16. Kerry-Williams, S. M., Noble, W. C. Group JK coryneform bacteria. Journal of Hospital Infection 1987, 9: 4–10.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. API/Bio Merieux Ltd., Grafton Way, RG22 6HY, Basingstoke, Hampshire, UK

    G. Tillotson

  2. Department of Clinical Microbiology, University College Hospital, Grafton Way, WC1E 6AU, London, UK

    M. Arora & M. Robbins

  3. Department of Medical Microbiology, Middlesex Hospital, Mortimer Street, W1N 8AA, London, UK

    J. Holton

Authors
  1. G. Tillotson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Arora
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Robbins
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Holton
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tillotson, G., Arora, M., Robbins, M. et al. Identification ofCorynebacterium jeikeium andCorynebacterium CDC group D 2 with the API 20 strep system. Eur. J. Clin. Microbiol. Infect. Dis. 7, 675–678 (1988). https://doi.org/10.1007/BF01964252

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01964252

Keywords

Search

Navigation