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Purification of 2-deoxy-2-dansylamido-D-glucose by affinity chromatography on a lectin-loaded agarose column

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Summary

A method is demonstrated to purify 2-substituted derivatives of glucose and sterically related sugars by affinity chromatography on Con A-sepharose. The method seems to be of rather general applicability.

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References

  1. H. Lis and N. Sharon, A. Rev. Biochem.42, 541 (1973).

    Article  CAS  Google Scholar 

  2. R. Axén, J. Porath and S. Ernback, Nature214, 1302 (1967).

    Article  PubMed  Google Scholar 

  3. J.P. Reeves, E. Shechter, R. Weil and H.R. Kaback, Proc. nat. Acad. Sci. USA70, 2722 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. S. Schuldinger, G.K. Kerwar and H.R. Kaback, J. biol. Chem.250, 1361 (1975).

    Article  Google Scholar 

  5. C. Gros and B. Labouesse, Eur. J. Biochem.7, 463 (1969).

    Article  CAS  PubMed  Google Scholar 

  6. Affinity chromatography, Principles and Methods: Pharmacia Fine Chemicals, Uppsala, Sweden 1974.

  7. I.J. Goldstein, C.M. Reichert, A. Misaki and P.A.J. Goren, Biochem. biophys. Acta317, 500 (1973).

    CAS  PubMed  Google Scholar 

  8. F.G. Loontiens, J.P. Van Wauwe, R. De Gussem and C.K. De Bruyne, Carbohydrate Res.30, 51 (1973).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Tübingen, Auf der Morgenstelle 28, D-74, Tübingen, Federal Republic of Germany

    W. Bessler & P. Schindler

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  1. W. Bessler
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  2. P. Schindler
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Bessler, W., Schindler, P. Purification of 2-deoxy-2-dansylamido-D-glucose by affinity chromatography on a lectin-loaded agarose column. Experientia 35, 1292–1293 (1979). https://doi.org/10.1007/BF01963964

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  • DOI: https://doi.org/10.1007/BF01963964

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