Antibody reactivity to cytomegalovirus structural polypeptides in subclinical and clinical human immunodeficiency virus infections

  • M. P. Landini
  • G. Mirolo
  • M. C. Re
  • A Ripalti
  • M. La Placa
Notes

Abstract

A total of 56 sera from asymptomatic HIV-seropositive subjects and patients with symptomatic HIV infection were studied by immunoblotting for IgM, IgG and IgG subclass reactivity against cytomegalovirus structural polypeptides. The results showed a high percentage of IgM-positive sera in the asymptomatic patients, and IgM reactivity was particularly high against a polypeptide of intermediate molecular weight (p66). IgG reactivity was very high against the majority of viral structural polypeptides with the possible exceptions of p82 and p61, in all patients. Antibody to these polypeptides decreased with the increase in HIV-specific symptoms. No significant difference in the reactivity of IgG subclass to viral poylpeptides was found among the different groups of subjects, except for a generalized increase in IgG subclass reactivity from asymptomatic to HIV-specific symptomatic patients.

Keywords

Molecular Weight Human Immunodeficiency Virus Virus Infection Polypeptide Human Immunodeficiency Virus Infection 

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References

  1. 1.
    Ho, M.: Cytomegalovirus biology and infection. In: Greenough, W. B. Merigan, T. C. (ed.). Plenum Press, New York, 1983, p. 79–104.Google Scholar
  2. 2.
    Hamilton, J. D.: Cytomegalovirus and immunity. In: Melnick, J. C. (ed.): Monographs in virology, Volume 12. S. Karger, Basel 1982, p. 8–78.Google Scholar
  3. 3.
    Plotkin, S. A., Michelson, S., Pagano, J., Rapp, F.: Cytomeganovirus: pathogenesis and prevention of human infection. (Birth defects: Original Articles Series, Volume 20), Alan R. Liss, New York, 1984, p. 10–230.Google Scholar
  4. 4.
    Quinnan, G. V., Masur, H., Rook, A. H., Amstrong, G., Frederick, W. R., Epstein, J., Manischewitz, M. S., Macher, M. D., Jackson, L., Ames, J., Smith, H. A., Parker, M., Pearson, G. R., Parillo, J., Mitchell, C., Strauss, S. E.: Herpesvirus infections in the acquired immune deficiency syndrome. Journal of the American Medical Association 1984, 252: 72–77.Google Scholar
  5. 5.
    Landini, M. P., Re, M. C., Mirolo, G., Baldassarri, B., La Placa, M.: Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. Journal of Medical Virology 1985, 17: 303–311.Google Scholar
  6. 6.
    Landini, M. P., Mirolo, G., Coppolecchia, P., Re, M. C., La Placa, M.: Serum antibodies to individual cytomegalovirus structural polypeptides in renal transplant recipients during viral infection. Microbiology and Immunology 1986, 30: 683–694.Google Scholar
  7. 7.
    Shimokawa, K., Murayama, T., Ishida, S., Furukawa, T.: Analysis by immunoblotting of human cytomegalovirus antibody in sera of renal transplant recipients. Journal of Medical Virology 1987, 21: 339–346.Google Scholar
  8. 8.
    Landini, M. P., Baldassarri, B., Mirolo, G., Ripalti, A., La Placa, M.: Reactivity of cytomegalovirus structural polypeptides with different subclasses of IgG present in human serum. Journal of Infection 1988, 16: 163–167.Google Scholar
  9. 9.
    Landini, M. P., Michelson, S.: Human cytomegalovirus proteins. Progress in Medical Virology 1988, 35: 152–185.Google Scholar
  10. 10.
    Burnette, W. N.: “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulphate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 1981, 112: 195–203.Google Scholar
  11. 11.
    Braun, D. J., Pereira, L., Norrild, B., Roizman, B.: Application of denatured, electrophoretically separated and immobilized ly sates of herpes simplex virus infected cells for detection of monoclonal antibodies and for the study of properties of viral proteins. Journal of Virology 1983, 46: 103–112.Google Scholar
  12. 12.
    Sundqvist, V. A., Linde, A., Kurth, R., Werner, A., Helm, E. B., Popovic, M., Gallo, R. C., Wahren, B.: Restricted IgG subclass response to HTLVIII/LAV and to cytomegalovirus in patients with AIDS and lymphadenopathy syndrome. Journal of Infectious Diseases 1986, 153: 970–973.Google Scholar
  13. 13.
    Rasmussen, L., Nelson, R. M., Kersall, D. C., Merigan, T. C.: Murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus. Proceedings of the National Academy of Sciences of the USA 1984, 81: 876–880.Google Scholar
  14. 14.
    Linde, G. A., Hammarstrom, L., Persson, M. A. A., Smith, C. I. E., Sundqvist, V. A., Wahren, B.: Virusspecific antibody activity of different subclasses of immunoglobulin G and A in cytomegalovirus infections. Infection and Immunity 1983, 42: 237–244.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • M. P. Landini
    • 1
  • G. Mirolo
    • 1
  • M. C. Re
    • 1
  • A Ripalti
    • 1
  • M. La Placa
    • 1
  1. 1.Institute of Microbiology, Medical FacultySt. Orsola General HospitalBolognaItaly

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