Abstract
The antibody response to P-fimbriae ofEscherichia coli in patients with upper urinary tract infections was investigated. In the sera of patients with pyelonephritis obtained at the initial visit to hospital (3 to 7 days after the onset of symptoms), a high incidence of antibodies to P-fimbriae was detected (12 out of 14 patients). P-fimbriatedEscherichia coli strains were isolated from urine samples in all of these antibody-positive patients. Antibodies detected by ELISA using purified antigen were essentially IgG and specifically recognized P-fimbriae. These antibodies inhibited completely, or in some cases partially, mannose-resistant hemagglutination with P-fimbriatedEscherichia coli.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Svanborg-Eden, C., Hansson, L. A., Jodal, U., Lindberg, U., Akerlund, A. S.: Variable adherence to normal human urinary-tract epithelial cells ofEscerichia coli strains associated with various forms of urinary-tract infection. Lancet 1976, ii: 490–492.
Källenius, G., Möllby, R., Winberg, J.: In vitro adhesion of uropathogenicEscherichia coli to human periurethral cells. Infection and Immunity 1980, 28: 972–980.
Svanborg-Eden, C., Hansson, H. A.:Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infection and Immunity 1978, 21: 229–237.
Källenius, G., Möllby, R., Svenson, S. B., Helin, I., Hultberg, H., Cedergren, B., Winberg, J.: Occurrence of P-fimbriatedEscherichia coli in urinary tract infections. Lancet 1981, ii: 1369–1372.
Jacobson, S. H., Lins, L. E., Svenson, S. B., Källenius, G.: P-fimbriatedEscherichia coli in adults with acute pyelonephritis. Journal of Infectious Disease 1985, 152: 426–427.
Källenius, G., Möllby, R., Svenson, S. B., Winberg, J., Hultberg, H.: Identification of a carbohydrate receptor recognized by uropathogenicEscherichia coli. Infection 1980, 8: 288–293.
Svanborg-Eden, C., Svennerholm, A. M.: Secretory immunoglobulin A and G antibodies prevent adhesion ofEscherichia coli to human urinary tract epithelial cells. Infection and Immunity 1978, 22: 790–797.
Rene, P., Dinolfo, M., Silverblatt, F. J.: Serum and urogenital antibody responses toEscherichia coli pili in cystitis. Infection and Immunity 1982, 38: 542–547.
de Ree, J. M., van den Bosch, J. F.: Serological response to the P-fimbriae of uropathogenicEscherichia coli in pyelonephritis. Infection and Immunity 1987, 55: 2204–2207.
Svenson, S. B., Källenius, G., Möllby, R., Hultberg, H., Winberg, J.: Rapid identification of P-fimbriatedEscherichia coli by a receptor-specific particle agglutination test. Infection 1982, 10: 209–214.
Korhonen, T. K., Nurmiaho, E. L., Ranta, H., Svanborg-Eden, C.: New method for isolation of immunologically pure pili fromEscherichia coli. Infection and Immunity 1980, 27: 569–575.
Dodd, D. C., Eisenstein, B. I.: Antigenic quantitation of type 1 fimbriae on the surface ofEscherichia coli cells by an enzyme-linked immunosorbent inhibition assay. Infection and Immunity 1982, 38: 764–773.
Lindberg, F., Lund, B., Johansson, L., Normark, S.: Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus. Nature 1987, 328: 84–87.
Ørskov, I., Ørskov, F.: The serology ofEscherichia coli fimbriae. Progress in Allergy 1983, 33: 80–105.
de Ree, J. M., Schwillens, P., van den Bosch, J. F.: Monoclonal antibodies that recognize the P-fimbriae F71, F72, F9, and F11 from uropathogenicEscherichia coli. Infection and Immunity 1985, 50: 900–904.
de Ree, J. M., Schwillens, P., van den Bosch, J. F.: Monoclonal antibodies for serotyping the P-fimbriae of uropathogenicEscherichia coli. Journal of Clinical Microbiology 1986, 24: 121–125.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Agata, N., Ohta, M., Miyazawa, H. et al. Serological response to P-fimbriae ofEscherichia coli in patients with urinary tract infections. Eur. J. Clin. Microbiol. Infect. Dis. 8, 156–159 (1989). https://doi.org/10.1007/BF01963903
Issue Date:
DOI: https://doi.org/10.1007/BF01963903