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Postantibiotic and bactericidal effect of imipenem againstPseudomonas aeruginosa

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Abstract

The postantibiotic effect of imipenem onPseudomonas aeruginosa was studied at different inocula using one ATCC strain and four clinical isolates. The postantibiotic effect was measured using two different methods: viable counts and bioluminescence assay of intracellular bacterial ATP. The postantibiotic effect could be demonstrated with both methods (viable counts 1–2 h, ATP assay 3–5 h) for all strains at an inoculum of 106 CFU/ml. When the inoculum was raised to 108 CFU/ml, no postantibiotic effect could be observed with either method using routine growth conditions. This disappearance of the postantibiotic effect coincided with a loss of bactericidal effect of imipenem when high inocula were used. Improved oxygenation of the cultures restored the bactericidal and postantibiotic effects of imipenem at high inocula.

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Authors and Affiliations

  1. Department of Infectious Diseases and Department of Clinical Bacteriology, Akademiska sjukhuset, Uppsala University, S-751 85, Uppsala, Sweden

    I. Odenholt & O. Cars

  2. Department of Clinical Bacteriology, Linköping University, Linköping, Sweden

    B. Isaksson & L. Nilsson

Authors
  1. I. Odenholt
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  2. B. Isaksson
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  3. L. Nilsson
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  4. O. Cars
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Odenholt, I., Isaksson, B., Nilsson, L. et al. Postantibiotic and bactericidal effect of imipenem againstPseudomonas aeruginosa . Eur. J. Clin. Microbiol. Infect. Dis. 8, 136–141 (1989). https://doi.org/10.1007/BF01963897

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