Abstract
The performance of two new commercial assays for the serological diagnosis ofMycoplasma pneumoniae infection (microparticle agglutination and antibody-capture enzyme-immunoassay) was studied using a panel of 169 serum samples from patients withMycoplasma pneumoniae pneumonia and a control group. Both assays were shown to be sensitive and specific for diagnosis. The performance of the capture immunoassay, however, decreased in older patients, probably due to its inability to detect cases of reinfection without IgM antibody response.
Similar content being viewed by others
References
Levine DP, Lerner AM: The clinical spectrum ofMycoplasma pneumoniae infections. Medical Clinics of North America 1978, 62: 961–978.
Vikerfors T, Brodien G, Grandien M, Hirschberg L, Krook A, Petterson C-A: Detection of specific IgM antibodies for the diagnosis ofMycoplasma pneumoniae infections: a clinical evaluation. Scandinavian Journal of Infectious Diseases 1988, 20: 601–610.
Brunner H, Prescott B, Greenberg H, James WD, Horswood RL, Chanock RM: Unexpected high frequency of antibody toMycoplasma pneumoniae in human sera as measured by sensitive techniques. Journal of Infectious Diseases 1977, 135: 524–530.
Wreghitt TG, Sillis M: A μ-capture ELISA for detectingMycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA. Journal of Hygiene 1985, 94: 217–227.
Moule JH, Caul EO, Wreghitt TG: The specific IgM response toMycoplasma pneumoniae infection: interpretation and application to early diagnosis. Epidemiology and Infection 1987, 99: 685–692.
Hirschberg L, Krook A, Petterson CA, Vikerfors T: Diagnosis ofMycoplasma pneumoniae infection by the detection of specific IgM with a μ-capture ELISA. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 420–423.
Grist NR, Ross CA, Bell EJ: Diagnostic methods in clinical virology. Blackwell Scientific Publications, Oxford, 1974.
Palmer DE, Cavallaro JJ, Hermann KL: Rubella hemagglutination inhibition test. Immunology series no. 2. Centers for Disease Control, Atlanta, 1977.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Echevarría, J.M., León, P., Balfagón, P. et al. Diagnosis ofMycoplasma pneumoniae infection by microparticle aggluination and antibody-capture enzyme-immunoassay. Eur. J. Clin. Microbiol. Infect. Dis. 9, 217–220 (1990). https://doi.org/10.1007/BF01963842
Issue Date:
DOI: https://doi.org/10.1007/BF01963842