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Evaluation of the microplate hybridization method for rapid identification ofLegionella species

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Abstract

Rapid genetic identification of 23Legionella species was performed using microdilution plate hybridization. When an isolate appeared to be a strain of theLegionella species, its DNA was quickly extracted, labelled with photobiotin and distributed to microdilution wells where 23 different DNAs had previously been immobilized. After hybridization for 90 min at 45 °C, the microdilution well which emitted the maximum fluorescence was determined. The criterion for interpreting results of this genetic identification method is given.

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Authors and Affiliations

  1. Department of Microbiology, Gifu University School of Medicine, 40 Tsukasa-machi, 500, Gifu, Japan

    T. Ezaki, Y. Hashimoto, H. Yamamoto, M. L. Lucida, S. L. Liu & E. Yabuuchi

  2. Research and Development Center, Kobayashi Pharmaceutical Co., 06, Osaka, Japan

    S. Kusunoki & K. Asano

Authors
  1. T. Ezaki
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  2. Y. Hashimoto
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  3. H. Yamamoto
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  4. M. L. Lucida
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  5. S. L. Liu
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  6. S. Kusunoki
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  7. K. Asano
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  8. E. Yabuuchi
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Ezaki, T., Hashimoto, Y., Yamamoto, H. et al. Evaluation of the microplate hybridization method for rapid identification ofLegionella species. Eur. J. Clin. Microbiol. Infect. Dis. 9, 213–217 (1990). https://doi.org/10.1007/BF01963841

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  • DOI: https://doi.org/10.1007/BF01963841

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