Abstract
The in vitro bactericidal activity of amoxicillin, gentamicin, rifampicin, ciprofloxacin and trimethoprim-sulfamethoxazole alone or in combination was determined against seven strains ofListeria monocytogenes by the killing curve method. Amoxicillin plus gentamicin was the most rapidly bactericidal combination, whereas trimethoprim-sulfamethoxazole was less bactericidal at 6 h but as bactericidal at 24 h. The combination of trimethoprim-sulfamethoxazole with either amoxicillin, ciprofloxacin or rifampicin did not result in antagonism, but the combinations were no more active than trimethoprim-sulfamethoxazole alone. The interaction of amoxicillin with rifampin was fairly antagonistic (1 log10 difference). The combination of amoxicillin and ciprofloxacin, although producing antagonism during the first 6 h, was more active at 24 h than amoxicillin alone and prevented the regrowth observed with ciprofloxacin alone. Ciprofloxacin and rifampicin interacted antagonistically during the first 6 h, and the combination was not very bactericidal (3 log10) but prevented the emergence of mutants, as observed with each drug alone, when used at concentrations greater than the MICs for the strain tested. These regimens merit evaluation in in vivo models ofListeria monocytogenes meningitis.
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References
Moellering R, Medoff G, Leech I, Wennersten C, Kunz L: Antibiotic synergism againstListeria monocytogenes. Antimicrobial Agents and Chemotherapy 1972, I: 30–34.
Boisivon A: Action bactériostatique et bactéricide comparée des pénicillines, céphalosporines, aminosides et des associations ampicillin-gentamicine et triméthoprime-sulfaméthoxazole surListeria monocytogenes. Annales de Microbiologie 1980, 131 B: 267–276.
Prichard MG, Miles HM, Pavillard ER:Listeria meningitis in vitro sensitivities to cotrimoxazole, penicillins and gentamicin. Australian and New Zealand Journal of Medicine 1983, 13: 76–77.
Spitzer PG, Hammer SM, Karchmer AW: Treatment ofListeria monocytogenes infection with trimethoprim-sulfamethoxazole: case report and review of the literature. Reviews of Infectious Diseases 1986, 8, 3: 427–430.
Stahl JP, Gaillat J, Croize J, Bru JP, Carpentier F, Micoud M: Traitement des méningites àListeria monocytogenes par le cotrimoxazole seul. In: Trimethoprime et sulfamides. Edition Arnette, 1984, Paris, p. 177–179.
Tuazon CU, Shamsuddin D, Miller H: Antibiotic susceptibility and synergy of clinical isolates ofListeria monocytogenes. Antimicrobial Agents and Chemotherapy 1982, 21: 525–527.
Winslow DL, Steele ML:Listeria bacteremia and peritonitis associated with a peritoneo-venous shunt: successful treatment with sulfamethoxazole and trimethoprim. Journal of Infectious Diseases 1984, 149: 820.
Winslow DL, Damme J, Diechman E: Delayed bactericidal activity of beta-lactam antibiotics againstListeria monocytogenes: antagonism of chloramphenicol and rifampicin. Antimicrobial Agents and Chemotherapy 1983, 23: 555–558.
Scheld M: Evaluation of rifampicin and other antibiotics againstListeria monocytogenes in vitro and in vivo. Reviews of Infectious Diseases 1983, 5, Supplement 3: 593–597.
Wolff M, Boutron L, Singlas E, Clair B, Decazes JM, Régnier B: Penetration of ciprofloxacin into cerebrospinal fluid of patients with bacterial meningitis. Antimicrobial Agents and Chemotherapy 1987, 31: 899–902.
Scheld MW, Fletcher DD, Fink FN, Sande M: Response to therapy in an experimental rabbit model of meningitis due toListeria monocytogenes. Journal of Infectious Diseases 1979, 140: 287–294.
Hof H, Emmerling P: Murine model for therapy of listeriosis in the compromised host. Chemotherapy 1984, 30: 125–130.
Visher WA, Rominger C: Rifampicin against experimental listeriosis in the mouse. Chemotherapy 1978, 24: 104–111.
Desplaces N, Gutmann L, Carlet J, Guibert J, Acar JF: The new quinolones and their combinations with other agents for therapy of severe infections. Journal of Antimicrobial Chemotherapy 1986, 17, Supplement A: 25–29.
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Boisivon, A., Guiomar, C. & Carbon, C. In vitro bactericidal activity of amoxicillin, gentamicin, rifampicin, ciprofloxacin and trimethoprim-sulfamethoxazole alone or in combination againstListeria monocytogenes . Eur. J. Clin. Microbiol. Infect. Dis. 9, 206–209 (1990). https://doi.org/10.1007/BF01963839
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DOI: https://doi.org/10.1007/BF01963839