Experientia

, Volume 40, Issue 2, pp 227–228 | Cite as

A new continuous optical assay for isocitrate lyase

  • E. Giachetti
  • G. Pinzauti
  • P. Vanni
Short Communications

Summary

A new continuous optical assay method for isocitrate lyase is reported. This is a coupled assay which requires lactate dehydrogenase as an ancillary enzyme. The method yields linear data up to 0.12 units/ml. The assay is also suitable for crude extracts.

Keywords

Enzyme Lactate Crude Extract Lactate Dehydrogenase Lyase 

References

  1. 2.
    McFadden, B.A., and Howes, W.V., Analyt. Biochem.1 (1960) 240.CrossRefGoogle Scholar
  2. 3.
    Dixon, G.H., and Kornberg, H.L., Biochem. J.72 (1959) 3P.Google Scholar
  3. 4.
    Johanson, R.A., Hill, J.M., and McFadden, B.A., Biochim. biophys. Acta364 (1974) 327.PubMedGoogle Scholar
  4. 5.
    Vincenzini, M.T., Pinzauti, G., and Vanni, P., It. J. Biochem.29 (1981) 329.Google Scholar
  5. 6.
    Pinzauti, G., Giachetti, E., and Vanni, P., Int. J. Biochem.14 (1982) 267.CrossRefPubMedGoogle Scholar
  6. 7.
    Warren, W.A., J. biol. Chem.245 (1970) 1675.PubMedGoogle Scholar
  7. 8.
    Roche, T.E., Williams, J.O., and McFadden, B.A., Biochim. biophys. Acta206 (1970) 193.PubMedGoogle Scholar
  8. 9.
    Wilkinson, J.N., Biochem. J.80 (1961) 324.PubMedGoogle Scholar
  9. 10.
    Cleland, W.W., Adv. Enzymol.29 (1967) 1.PubMedGoogle Scholar
  10. 11.
    Segel, I.H., Enzyme kinetics. Wiley. New York 1975.Google Scholar

Copyright information

© Birkhäuser Verlag Basel 1984

Authors and Affiliations

  • E. Giachetti
    • 1
  • G. Pinzauti
    • 1
  • P. Vanni
    • 1
  1. 1.Istituto interfacoltà di Chimica biologicaUniversità di FirenzeFirenze(Italy)

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