Monoclonal antibodies to L-asparaginase

Summary

Five hybridomas secreting monoclonal antibody toE. coli L-asparaginase were isolated. These monoclonal antibodies were classified into 3 different subclasses; Ig G₁ (1 clone), Ig G₂ (2 clones) and Ig G₃ (2 clones). One of them possessed anti-L-asparaginase neutralizing activity. Four antibodies examined demonstrated a linear Langmuir binding plot and binding affinities, with equilibrium dissociation constant (K_d) ranging between 2.5×10⁻⁹M and 6.3×10⁻¹⁰ M. The monoclonal antibodies should be useful probes for investigation of the enzyme activity.