, Volume 40, Issue 2, pp 193–195 | Cite as

Physiological state of submitochondrial particles and their susceptibility to Triton X-100

  • F. M. Goñi
  • J. M. Valpuesta
  • M. C. Barbero
  • E. Rial
  • J. I. G. Gurtubay
  • J. M. Macarulla
Short Communications


The solubilizing effect of Triton X-100 on beef heart submitochondrial particles (ETPH) has been studied under various physiological conditions. Coupled, uncoupled and azide-inhibited ETPH particles have been studied. Quantitative and qualitative differences are found in the proteins solubilized by the detergent from ETPH particles under the various conditions tested.


Physiological Condition Qualitative Difference Beef Heart Solubilizing Effect Submitochondrial Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 2.
    Cheah, K.S., Biochim. biophys. Acta180 (1969) 320.PubMedGoogle Scholar
  2. 3.
    Lanyi, J.K., Biochemistry12 (1973) 1433.CrossRefPubMedGoogle Scholar
  3. 4.
    Komor, E., Weber, H., and Tanner, W., Proc. natl Acad. Sci. USA76 (1979) 1814.PubMedGoogle Scholar
  4. 5.
    Egan, R.W., Jones, M.A., and Lehninger, A.L., J. biol. Chem.251 (1976) 4442.PubMedGoogle Scholar
  5. 6.
    Gurtubay, J.I.G., Goñi, F.M., Gómez-Fernández, J.C., Otamendi, J.J., and Macarulla, J.M., J. Biomembrane Bioenerg.12 (1980) 47.CrossRefGoogle Scholar
  6. 7.
    Barbero, M.C., Rial, E., Otamendi, J.J., Gurtubay, J.I.G., and Goñi, F.M., Int. J. Biochem.14 (1982) 933.CrossRefPubMedGoogle Scholar
  7. 8.
    Hackenbrock, C.R., J. Cell Biol.37 (1968) 345.CrossRefPubMedGoogle Scholar
  8. 9.
    Helenius, A., and Simons, K., Biochim. biophys. Acta415 (1975) 29.PubMedGoogle Scholar
  9. 10.
    Bevers, E.M., Leblanc, G., Le Grimellec, C., Op den Kamp, J.A.F., and Van Deenen, L.L., FEBS Lett.87 (1978) 49.CrossRefPubMedGoogle Scholar
  10. 11.
    Gazzit, Y., Loyter, A., Reichler, Y., and Ohad, I., Biochim. biophys. Acta419 (1976) 479.PubMedGoogle Scholar
  11. 12.
    Nieva-Gómez, D., and Gennis, R.B., Proc. natl Acad. Sci. USA74 (1977) 1811.PubMedGoogle Scholar
  12. 13.
    Low, H., and Vallin, I., Biochim. biophys. Acta69 (1963) 361.CrossRefGoogle Scholar
  13. 14.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. biol. Chem.193 (1951) 265.PubMedGoogle Scholar
  14. 15.
    Wang, C.S., and Smith, R.L., Analyt. Biochem.63 (1975) 414.CrossRefPubMedGoogle Scholar
  15. 16.
    Fairbanks, G., Steck, T.L., and Wallach, D.F.H., Biochemistry10 (1971) 2605.CrossRefGoogle Scholar
  16. 17.
    Ferguson, S.J., Lloyd, W.J., and Radda, G.K., Biochim. biophys. Acta423 (1976) 174.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1984

Authors and Affiliations

  • F. M. Goñi
    • 1
  • J. M. Valpuesta
    • 1
  • M. C. Barbero
    • 1
  • E. Rial
    • 1
  • J. I. G. Gurtubay
    • 1
  • J. M. Macarulla
    • 1
  1. 1.Departments of Biochemistry and BiologyFaculty of ScienceBilbao(Spain)

Personalised recommendations