Advertisement

Experientia

, Volume 37, Issue 2, pp 122–123 | Cite as

A comment on the design of experiments to estimate the Michaelis-Menten parameters of enzyme-catalysed reactions

  • G. L. Atkins
  • I. A. Nimmo
Specialia

Summary

Michaelis-Menten parameters can be estimated by measuring initial velocities (v) in replicate at 2 concentrations of substrate, one much lower than Km and the other much higher than it. Analysis of simulated experimental data suggests that this design will probably give more precise estimates of Km and V than the conventional design in which v is measured at several different concentrations of substrate.

Keywords

Experimental Data Initial Velocity Precise Estimate Conventional Design Simulated Experimental Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    I. A. Nimmo and G. L. Atkins, Trends biochem. Sci.4, 236 (1979).CrossRefGoogle Scholar
  2. 2.
    A. Cornish-Bowden, in: Principles of Enzyme Kinetics, p. 177. Butterworths, London 1976.Google Scholar
  3. 3.
    A. Cornish-Bowden and R. Eisenthal, Biochim. biophys. Acta523, 268 (1978).PubMedGoogle Scholar
  4. 4.
    G. L. Atkins and I. A. Nimmo, Analyt. Biochem.104, 1 (1980).CrossRefPubMedGoogle Scholar
  5. 5.
    S. F. Mabood, P. F. J. Newman and I. A. Nimmo, Biochem. Soc. Trans.7, 924 (1979).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • G. L. Atkins
    • 1
  • I. A. Nimmo
    • 1
  1. 1.Department of BiochemistryUniversity of Edinburgh Medical SchoolEdinburgh(Scotland)

Personalised recommendations