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Experientia

, Volume 39, Issue 4, pp 347–350 | Cite as

Modulation of protein synthesis in primary myogenic cells from chicken by cultivation in the serum-free, hormonally defined medium ‘DMN’

  • P. Dollenmeier
  • H. M. Eppenberger
Full Papers

Summary

Chicken muscle cells secrete characteristic proteins when grown in the serum-free and hormonally defined culture medium ‘DMN’. The most prominent band detected by gel electrophoresis represents a protein of mol.wt 22,000. Fibroblasts released a mol.wt 16,000 protein and fibronectin (mol.wt 220,000) into the medium. The mol.wt 22,000 protein band resolved in 2 dimensional gels into 2 spots which migrated to the same positions as small heat shock proteins as well as butyrate-inducible proteins (BIP) which can be demonstrated in whole cell extracts after butyrate treatment in the presence of serum. The synthesis and release of the mol.wt 22,000 protein is repressed by supplementing the culture medium with serum but not with chick embryo extract.

Keywords

Muscle Cell Electrophoresis Protein Synthesis Heat Shock Heat Shock Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 4.
    Ashburner, M., and Bonner, J. J., The induction of gene activity inDrosophila by heat shock. Cell17 (1979) 241–254.PubMedGoogle Scholar
  2. 5.
    Kelley, P. M., Aliperti, G., and Schlesinger, M. J., In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts. J. biol. Chem.255 (1978) 1277–1286.Google Scholar
  3. 6.
    Key, J. L., Lin, C. Y., and Chen, M. Y., Heat shock proteins of higher plants. Proc. natl Acad. Sci. USA78 (1981) 3526–3530.Google Scholar
  4. 7.
    Storti, R. V., Scott, M., Rich, A., and Pardue, M. L., Translational control of protein synthesis in response to heat shock inD. melanogaster cells. Cell22 (1980) 825–834.PubMedGoogle Scholar
  5. 8.
    Johnston, D., Oppermann, H., Jackson, J., and Levinson, W., Induction of four proteins in chick embryo cells by sodium arsenite. J. biol. Chem.255 (1980) 6975–6980.Google Scholar
  6. 9.
    Kelley, P. M., and Schlesinger, M. J., The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts. Cell15 (1978) 1277–1286.PubMedGoogle Scholar
  7. 10.
    Levinson, W., Oppermann, H., and Jackson, J., Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells. Biochim. biophys. Acta606r (1980) 170–180.Google Scholar
  8. 11.
    Wang, C., Gomer, R. H., and Lazarides, E., Heat shock proteins are methylated in avian and mammalian cells. Proc. natl Acad. Sci. USA78 (1981) 3531–3535.PubMedGoogle Scholar
  9. 12.
    Levinger, L., and Varshavsky, A., Heat shock proteins ofDrosophila are associated with nuclease-resistant, highsaltresistant nuclear structures. J. Cell Biol.90 (1981) 793–796.PubMedGoogle Scholar
  10. 13.
    Velasquez, J. M., DiDomenico, B. J., and Lindquist, S., Intracellular localization of heat shock proteins inDrosophila. Cell20 (1980) 679–689.PubMedGoogle Scholar
  11. 14.
    Dollenmeier, P., Turner, D. C., and Eppenberger, H. M., Proliferation and differentiation of chick skeletal muscle cell cultures in a chemically defined medium. Exp. Cell Res.135 (1981) 47–61.PubMedGoogle Scholar
  12. 15.
    Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227 (1970) 600–685.Google Scholar
  13. 16.
    O'Farrell, P. H., High resolution two-dimensional electrophoresis of proteins. J. biol. Chem.250 (1975) 4007–4021.PubMedGoogle Scholar
  14. 17.
    Caravatti, M., Perriard, J. C., and Eppenberger, H. M., Developmental regulation of creatine kinase isoenzymes in myogenic cell cultures from chicken. J. biol. Chem.254 (1979) 1388–1394.PubMedGoogle Scholar
  15. 18.
    Mosher, D. F., Fibronectin. Prog. Hemostasis Thromb.5 (1980) 111–151.Google Scholar
  16. 19.
    Devlin, R. B., and Emerson, C. P., Coordinate regulation of contractile protein synthesis during myoblast differentiation. Cell13 (1978) 599–611.PubMedGoogle Scholar
  17. 20.
    Minty, C., Montarras, D., Fiszman, M. Y., and Gros, F., Butyrate-treated chick embryo myoblasts synthesize new proteins. Exp. Cell Res.133 (1981) 63–72.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • P. Dollenmeier
    • 1
  • H. M. Eppenberger
    • 1
  1. 1.Institute for Cell BiologySwiss Federal Institute of TechnologyZürich(Switzerland)

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