Abstract
A system for typingNeisseria meningitidis has been developed which uses monoclonal antibodies against two separate classes of outer membrane proteins (class 1 and class 2/3) in addition to capsular polysaccharide serogrouping. It was shown that class 1 outer membrane protein subtypes are common to meningococcal reference strains of different serotypes. Application of the system to 50 group B meningococcal patient isolates revealed that 75 % could be categorized as class 1 subtypes. The typing system has potential usefulness in epidemiological surveillance and vaccine development.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lynch, E. C., Blake, M. S., Gotschlich, E. C., Mauro, A. Studies on porins spontaneously transferred from whole cells and reconstituted from purified proteins ofNeisseria gonorrhoeae andNeisseria meningitidis. Biophysical Journal 1984, 45: 104–107.
Gotschlich, E. C., Seiff, M., Blake, M. S. The DNA sequence of the structural gene of gonococcal protein III and the flanking region containing a repetitive sequence. Journal of Experimental Medicine 1987, 165: 471–482.
King, G. J., Swanson, J. Studies on gonococcus infection. XV: Identification of surface proteins ofNeisseria gonorrhoeae correlated with leukocyte association. Infection and Immunity 1978, 11: 575–584.
Poolman, J. T., Wientjes, F. B., Hopman, C. T. P., Zanen, H. C. Influence of the length of LPS molecules on the surface exposure of class 1 and 2 outer membrane proteins ofNeisseria meningitidis strain 2996 (B: 2b: P1.2). In: Schoonik, G. K. (ed.): PathogenicNeisseria. American Society for Microbiology, Washington, DC, 1985, p. 562–570.
Frasch, C. E., Zollinger, W. D., Poolman, J. T. Serotype antigens ofNeisseria meningitidis and a proposed scheme for designation of serotypes. Reviews of Infectious Diseases 1985, 7: 504–510.
Poolman, J. T., Hopman, C. T. P., Zanen, H. C. Colony variants ofNeisseria meningitidis strain 2996 (B:2b:P1.2). Influence of class 5 outer membrane proteins and lipopolysaccharides. Journal of Medical Microbiology 1985, 12: 203–209.
Abdillahi, H., Crowe, B. A., Achtman, M., Poolman, J. T. Two monoclonal antibodies specific for serotype 4 antigens ofNeisseria meningitidis. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 293–296.
Poolman, J. T., Buchanan, T. M. Monoclonal antibody activity against native and denatured forms of gonococcal outer membrane proteins as detected within ultrathin, longitudinal slices of polyacrylamide gels. Journal of Immunological Methods 1984, 75: 265–274.
Poolman, J. T., Lind, I., Jonsdottir, K., Froholm, L. O., Jones, D. M., Zanen, H. C. The occurrence of serotypes 2b and 15 among group B meningococci in North-West Europe. Lancet, 1986, ii: 555–558.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Poolman, J.T., Abdillahi, H. Outer membrane protein serosubtyping ofNeisseria meningitidis . Eur. J. Clin. Microbiol. Infect. Dis. 7, 291–292 (1988). https://doi.org/10.1007/BF01963104
Issue Date:
DOI: https://doi.org/10.1007/BF01963104