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Typing ofStreptococcus agalactiae (Lancefield group B)

  • G. Colman
Current Topic: Review

Abstract

A two-stage typing scheme in routine use in this laboratory is described. The strains of group B streptococci (GBS) are first serotyped and then, if necessary, phage-typing is performed. Serotyping lacks discrimination because almost 40% of strains handled carry either the Ia/c or III/R antigens. On the other hand, the typability rate with phages is less than 80%. The evidence from the combined scheme is consistent with the view that GBS infections occurring within the first five days of life are, with few exceptions, due to acquisition of the mother's flora. After this time the infecting strain may come from other sources. Human and bovine strains of GBS belong predominantly to two recognisably different populations that can be distinguished by antigenic and biochemical differences. There are no patterns of lysis by phages characteristic of either human or bovine strains.

Keywords

Internal Medicine Typability Rate Typing Scheme Combine Scheme Biochemical Difference 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Minett, F.C. The bacteriology of bovine streptococcal mastitis. Journal of Hygiene 1935, 35: 504–511.Google Scholar
  2. 2.
    Lancefield, R.C. A serological differentiation of human and other groups of hemolytic streptococci. Journal of Experimental Medicine 1933, 57: 571–595.Google Scholar
  3. 3.
    Hare, R., Colebrook, L. The biochemical reactions of haemolytic streptococci from the vagina of febrile and afebrile parturient women. Journal of Pathology and Bacteriology 1934, 39: 429–442.Google Scholar
  4. 4.
    Hood, M., Janney, A., Dameron, G. Beta hemolytic streptococcus group B associated with problems of the perinatal period. American Journal of Obstetrics and Gynecology 1961, 82: 809–818.Google Scholar
  5. 5.
    Brown, J.H. Classification of streptococci, groups A, B, C and D. International Bulletin of Bacteriological Nomenclature and Taxonomy 1953, 3: 163–169.Google Scholar
  6. 6.
    Butter, M.N.W., de Moor, C.E. Streptococcus agalactiae as a cause of meningitis in the newborn, and of bacteraemia in adults. Antonie van Leeuwenhoek 1967, 33: 439–450.Google Scholar
  7. 7.
    Lancefield, R.C. Loss of the properties of hemolysin and pigment production without change in immunological specificity in a strain ofStreptococcus haemolyticus. Journal of Experimental Medicine 1934, 59: 459–469.Google Scholar
  8. 8.
    Islam, A.K.M.S. Rapid recognition of group-B streptococci. Lancet 1977, i: 256–257.Google Scholar
  9. 9.
    Merritt, K., Jacobs, N.J. Characterization and incidence of pigment production by human clinical group B streptococci. Journal of Clinical Microbiology 1978, 8: 105–107.Google Scholar
  10. 10.
    Wanger, A.R., Dunny, G.M. Development of a system for genetic and molecular analysis ofStreptococcus agalactiae. Research in Veterinary Science 1985, 38: 202–208.Google Scholar
  11. 11.
    Lancefield, R.C. A serological differentiation of specific types of bovine hemolytic streptococci (group B). Journal of Experimental Medicine 1934, 59: 441–458.Google Scholar
  12. 12.
    Lancefield, R.C. Two serological types of group B hemolytic streptococci with related, but not identical, type-specific substances. Journal of Experimental Medicine 1938, 67: 25–40.Google Scholar
  13. 13.
    Kasper, D.L., Jennings, H.J. Immunological, immunochemical and structural studies of the type III and Ia group B streptococcal polysaccharides. In: Easmon, C.S.F., Jeljaszewicz, J. (ed.): Medical microbiology, Volume 1. Academic Press, London, 1982, p. 183–216.Google Scholar
  14. 14.
    Jennings, H.J., Rosell, K.G., Katzenellenbogen, E., Kasper, D.L. Structural determination of the capsular polysaccharide antigen of type II group B streptococcus. Journal of Biological Chemistry 1983, 258: 1793–1798.Google Scholar
  15. 15.
    Pattison, I.H., Matthews, P.R.J., Howell, D.G. The type classification of group-B streptococci, with special reference to bovine strains apparently lacking in type polysaccharide. Journal of Pathology and Bacteriology 1955, 69: 51–60.Google Scholar
  16. 16.
    Wilkinson, H.W., Eagon, R.G. Type-specific antigens of group B type Ic streptococci. Infection and Immunity 1971, 4: 596–604.Google Scholar
  17. 17.
    Bevanger, L. Ibc proteins as serotype markers of group B streptococci. Acta Pathologica et Microbiologica Scandinavica (B) 1983, 91: 231–234.Google Scholar
  18. 18.
    Stableforth, A.W. Serological classification of the mastitis streptococci. In: Little, R.B., Plastridge, W.N. (ed.): Bovine mastitis. McGraw-Hill, New York, 1946, p. 203–245.Google Scholar
  19. 19.
    Pattison, I.H., Matthews, P.R.J., Maxted, W.R. Type classification by Lancefield's precipitin method of human and bovine group-B streptococci isolated in Britain. Journal of Pathology and Bacteriology 1955, 59: 43–50.Google Scholar
  20. 20.
    Jelinkova, J., Motlova, J. Worldwide distribution of two new serotypes of group B streptococci: type IV and provisional type V. Journal of Clinical Microbiology 1985, 21: 361–362.Google Scholar
  21. 21.
    Straus, D.C., Yeung, M.K., Durham, D.L. Mattingly, S.J. Lack of virulence of bovine type IIIStreptococcus agalactiae strains for mice correlates with reduced in vitro production of extracellular type-specific antigen. Current Microbiology 1982, 7: 251–256.Google Scholar
  22. 22.
    Stringer, J., Crees-Morris, J.A., Young, S.E.J., Mayon-White, R.T. Group B streptococcal systemic disease in Great Britain. Journal of Infection 1981, 3: 385–391.Google Scholar
  23. 23.
    Wilkinson, H.W. Group B streptococcal infection in humans. Annual Review of Microbiology 1978, 32: 41–57.Google Scholar
  24. 24.
    Jelinkova, J. Group B streptococci in the human population. Current Topics in Microbiology and Immunology 1977, 76: 127–165.Google Scholar
  25. 25.
    Stringer, J. Phage typing ofStreptococcus agalactiae. In: Bergan, T. (ed.): Methods in microbiology. Volume 16. Academic Press, London, 1984, p. 1–22.Google Scholar
  26. 26.
    Gray, B.M., Pass, M.A., Dillon, H.C. Laboratory and field evaluation of selective media for isolation of group B streptococci. Journal of Clinical Microbiology 1979, 9: 466–470.Google Scholar
  27. 27.
    Easmon, C.S.F., Hastings, M.J.G., Blowers, A., Bloxham, B., Deeley, J., Marwood, R., Rivers, R.P.A., Stringer, J. Epidemiology of group B streptococci: one year's experience in an obstetric and special care baby unit. British Journal of Obstetrics and Gynaecology 1983, 90: 241–246.Google Scholar
  28. 28.
    Hastings, M.J.G., Easmon, C.S.F., Neill, J., Bloxham, B., Rivers, R.P.A. Group B streptococcal colonisation and the outcome of pregnancy. Journal of Infection 1986, 12: 23–29.Google Scholar
  29. 29.
    Ross, P.W., Neilson, J.R. Group B streptococci in mothers and infants: Edinburgh studies. Health Bulletin 1982, 40: 234–239.Google Scholar
  30. 30.
    Duben, J., Jelinkova, J., Neubauer, M. Group B streptococci in the female genital tract and nosocomial colonization of newborns. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (A) 1978, 242: 168–180.Google Scholar
  31. 31.
    Weindling, A.M., Hawkins, J.M., Coombes, M.A., Stringer, J. Colonisation of babies and their families by group B streptococci. British Medical Journal 1981, 283: 1503–1505.Google Scholar
  32. 32.
    Boyer, K.M., Gadzala, C.A., Burd, L.I., Fisher, D.E., Paton, J.B., Gotoff, S.P. Selective intrapartum chemoprophylaxis of neonatal group B streptococcal early-onset disease. I. Epidemiologic rationale. Journal of Infectious Diseases 1983, 148: 795–800.Google Scholar
  33. 33.
    Cartensen, H., Henrichsen, J., Jepsen, O.B. A national survey of severe group B streptococcal infections in neonates and young infants in Denmark, 1978–1983. Acta Paediatrica Scandinavica 1985, 74: 934–941.Google Scholar
  34. 34.
    Mulder, C.J.J., Zanen, H.C. Neonatal group B streptococcal meningitis. Archives of Disease in Childhood 1984, 59: 439–443.Google Scholar
  35. 35.
    Mayon-White, R.T. The incidence of GBS disease in neonates in different countries. Antibiotics and Chemotherapy 1985, 35: 17–27.Google Scholar
  36. 36.
    Mayon-White, R.T. The incidence of neonatal group-B streptococcal disease in Britain. In: Holm, S.E., Christensen, P. (ed.): Basic concepts of streptococci and streptococcal diseases. Reedbooks, Chertsey, 1982, p. 305–306.Google Scholar
  37. 37.
    Baker, C.J. Group B streptococcal infections. In: Stollerman, G.H. (ed.): Advances in internal medicine. Year Book Medical Publishers, Chicago, 1980, p. 475–501.Google Scholar
  38. 38.
    Weems, J.J., Jarvis, W.R., Colman, G. A cluster of late onset group B streptococcal infections in low birthweight premature infants: no evidence for horizontal transmission. Pediatric Infectious Disease 1986, 5: 715–717.Google Scholar
  39. 39.
    Easmon, C.S.F., Hastings, M.J.G., Clare, A.J., Bloxham, B., Marwood, R., Rivers, R.P.A., Stringer, J. Nosocomial transmission of group B streptococci. British Medical Journal 1981, 283: 459–461.Google Scholar
  40. 40.
    Brglez, I., Jelinkova, J., Zvokelj-Krizan, B., Kastelic, M. Findings of serotypes of group B streptococci in human and bovine sources. Journal of Hygiene, Epidemiology, Microbiology and Immunology 1979, 23: 155–158.Google Scholar
  41. 41.
    Brglez, I., Stropnik, Z., Batis, J. Phagetypes of human and bovineStreptococcus agalactiae in Slovenia. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (B) 1986, 182: 401–406.Google Scholar
  42. 42.
    Baker, C.J. Nosocomial septicemia and meningitis in neonates. American Journal of Medicine 1981, 70: 698–701.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • G. Colman
    • 1
  1. 1.Central Public Health LaboratoryDivision of Hospital InfectionLondonUK

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