Abstract
Two hundred and twenty-threeHaemophilus influenzae strains isolated from patients with otitis media throughout France were characterized by biotype, serotype, antibiotic susceptibility, type of beta-lactamase production and human erythrocyte agglutination properties. All strains fell in one of two groups. One group consisted of encapsulated type b strains, 50% of which were biotype I, often resistant to ampicillin (38.5% of beta-lactamase producing strains) and seldom positive for haemagglutination (3.8%). The second group was composed of non-encapsulated strains, 42% of which were of biotype II, 10.6% beta-lactamase producers and 10.5% positive for haemagglutination.
Similar content being viewed by others
References
Bluestone, C.D. Recent advances in the pathogenesis, diagnosis, and management of otitis media. Pediatric Clinics of North America 1981, 28: 727–755.
Harding, A.L., Anderson, P., Howie, V.M., Ploussard, J.H., Smith, D.H. Hemophilus influenzae isolated from children with otitis media. In: Sell, S.H.W., Karzon, D.T. (ed.):Hemophilus influenzae. Vanderbilt University Press, Nashville, Tennessee, 1973, p. 21–28.
Van Hare, G.F., Shurin, P.A., Marchant, C.D., Cartelli, N.A., Johnson, C.E., Fulton, D., Carlin, S., Kim, C.H. Acute otitis media caused byBranhamella catarrhalis: biology and therapy. Reviews of Infectious Diseases 1987, 9: 16–27.
Turk, D.C. The pathogenicity ofHaemophilus influenzae. Journal of Medical Microbiology 1984, 18: 1–6.
Murphy, T.F., Apicella, M.A. NontypableHaemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Reviews of Infectious Diseases 1987, 9: 1–15.
Moxon, E.R. The carrier state:Haemophilus influenzae. Journal of Antimicrobial Chemotherapy 1986, 18, Supplement A: 17–24.
Kilian, M., Heine-Jensen, J., Bulow, P. Haemophilus in the upper respiratory tract of children: a bacteriological, serological and clinical investigation. Acta Pathologica Microbiologica Scandinavia (B) 1972, 80: 571–578.
Kilian, M. A taxonomic study of the genusHaemophilus, with the proposal of a new species. Journal of General Microbiology 1976, 93: 9–62.
Granato, P.A., Jurek, E.A., Weiner, L.B. Biotypes ofHaemophilus influenzae: relationship to clinical source of isolation, serotype, and antibiotic susceptibility. American Journal of Clinical Pathology 1983, 79: 73–77.
Kilian, M., Sorensen, I., Frederiksen, W. Biochemical characteristics of 130 recent isolates fromHaemophilus influenzae meningitis. Journal of Clinical Microbiology 1979, 9: 409–412.
Albritton, W.L., Penner, S., Slaney, L., Brunton, J. Biochemical characteristics ofHaemophilus influenzae in relationship to source of isolation and antibiotic resistance. Journal of Clinical Microbiology 1978, 7: 519–523.
Oberhofer, T.R., Back, A.E. Biotypes ofHaemophilus encountered in clinical laboratories. Journal of Clinical Microbiology 1979, 10: 168–174.
Demaria, T.F., Lim, D.J., Barnishan, J., Ayers, L.W., Birck, H.G. Biotypes of serologically nontypableHaemophilus influenzae isolated from the middle ears and nasopharynges of patients with otitis media with effusion. Journal of Clinical Microbiology 1984, 20: 1102–1104.
Pichichero, M.E., Anderson, P., Loeb, M., Smith, D.H. Do pili play a role in pathogenicity ofHaemophilus influenzae type b? Lancet 1982, ii: 960–962.
Lampe, R.M., Mason, E.O., Kaplan, S.L., Umstead, C.L., Yow, M.D., Feigin, R.D. Adherence ofHaemophilus influenzae to buccal epithelial cells. Infection and Immunity 1982, 35: 166–172.
Porras, O., Svanborg-Eden, C., Lagergård, T., Hanson, L.Å. Method for testing adherence ofHaemophilus influenzae to human buccal epithelial cells. European Journal of Clinical Microbiology 1985, 4: 310–315.
Williams, J.D., Moosdeen, F. Antibiotic resistance inHaemophilus influenzae: epidemiology, mechanisms, and therapeutic possibilities. Reviews of Infectious Diseases 1986, 8 Supplement 5: S555-S561.
Farrar, W.E., O'Dell, N.M. Beta-lactamase activity in ampicillin-resistantHaemophilus influenzae. Antimicrobial Agents and Chemotherapy 1974, 6: 625–629.
Long, S.S., Teter, M.J., Gilligan, P.H. Biotype ofHaemophilus influenzae: correlation with virulence and ampicillin resistance. Journal of Infectious Diseases 1983, 147: 800–806.
Doern, G.V., Jorgensen, J.H., Thornsberry, C., Preston, D.A. theHaemophilus influenzae surveillance group: Prevalence of antimicrobial resistance among clinical isolates ofHaemophilus influenzae: a collaborative study. Diagnostic Microbiology and Infectious Diseases 1986, 4: 95–107.
Schwartz, R., Rodriguez, W., Khan, W., Ross, S. The increasing incidence of ampicillin-resistantHaemophilus influenzae. A cause of otitis media. Journal of the American Medical Association 1978, 239: 320–323.
Kilian, M. Haemophilus. In: Lennette, E.H., Balows, A., Hausler, W.J., Shadomy, H.J. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1985, p. 387–393.
Kilian, M., Biberstein, E.L. Genus II.Haemophilus. Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561AL. In: Krieg, N.R., Holt, J.G. (ed.): Bergey's manual of systematic bacteriology. Williams Wilkins, London, 1984, p. 558–569.
Sottnek, F.O., Albritton, W.L. Haemophilus influenzae biotype VIII. Journal of Clinical Microbiology 1984, 20: 815–816.
Courvalin, P., Goldstein, F., Phillipon, A., Sirot, J. L'antibiogramme. MPC — Vidéom, Paris, 1985, p. 297.
Ericsson, H.M., Sherris, J.C. Antibiotic sensitivity testing. Report of an International Collaborative Study. Acta Pathologica Microbiologica Scandinavia (B) 1971, Supplement 217: 9–90.
O'Callaghan, C.E., Morris, A., Kirby, S.M., Shingler, A.H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrobial Agents and Chemotherapy 1972, 1: 283–288.
Matthew, M., Harris, A.M., Marshall, M.J., Ross, G.W. The use of analytical isoelectric focusing for detection and identification of beta-lactamase. Journal of General Microbiology 1975, 88: 169–178.
Connor, E.M., Loeb M.R. A hemadsorption method for detection of colonies ofHaemophilus influenzae type b expressing fimbriae. Journal of Infectious Diseases 1983, 148: 855–860.
Medeiros, A.A., Levesque, R., Jacoby, G.A. An animal source for the ROB-1 beta-lactamase ofHaemophilus influenzae type b. Antimicrobial Agents and Chemotherapy 1986, 29: 212–215.
Mendelman, P.M., Chaffin, D.O., Stull, T.L., Rubens, C.E., Mack, K.D., Smith, A.L. Characterization of non-betalactamase-mediated ampicillin resistance inHaemophilus influenzae. Antimicrobial Agents and Chemotherapy 1984, 26: 235–244.
Dabernat, H., Delmas, C., Lareng, M.B. Prevalence of antimicrobial resistance amongHaemophilus influenzae isolated in France: a collaborative study. Pathologie Biologie 1986, 34: 372–378.
Pichichero, M.E. Adherence ofHaemophilus influenzae to human buccal and pharyngeal epithelial cells: relationship to piliation. Journal of Medical Microbiology 1984, 18: 107–116.
Mason, E.O., Kaplan, S.L., Wiedermann, B.L., Norrod, E.P., Stenback, W.A. Frequency and properties of naturally occurring adherent piliated strains ofHaemophilus influenzae type b. Infection and Immunity 1985, 49, 98–103.
Stull, T.L., Mendelman, P.M., Hass, J.E., Schoenborn, M.A., Mack, K.D., Smith, A.L. Characterization ofHaemophilus influenzae type b fimbriae. Infection and Immunity 1984, 46: 787–796.
Apicella, M.A., Shero, M., Dudas, K.C., Stack, R.R., Klohs, W., Lascolea, L.J., Murphy, T.F., Mylotte, J.M. Fimbriation ofHaemophilus species isolated from the respiratory tract of adults. Journal of Infectious Diseases 1984, 150: 40–43.
Kahn, M.E., Gromkova, R. Occurrence of pili on and adhesive properties ofHaemophilus parainfluenzae. Journal of Bacteriology 1981, 145: 1075–1078.
Kaplan, S.L., Mason, E.O., Wiedermann, B.L. Role of adherence in the pathogenesi ofHaemophilus influenzae type b infection in infant rats. Infection and Immunity 1983, 42: 612–617.
Anderson, P.W., Pichichero, M.E., Connor, E.M. Enhanced nasopharyngeal colonization of rats by piliatedHaemophilus influenzae type b. Infection and Immunity 1985, 48: 565–568.
Dabernat, H., Delmas, C., Lareng, M.G. Changes in susceptibility to antibiotics ofHaemophilus (H. influenzae andH. parainfluenzae) strains recovered from the upper respiratory tract in children. Pathologie Biologie 1985, 33: 391–395.
Salit, I.E., Morton, G. Adherence ofNeisseria meningitidis to human epithelial cells. Infection and Immunity 1981, 31: 430–435.
Andersson, B., Falsen, B.E. Fogh, A., Hanson, L.Å, Nylén, O., Peterson, H., Svanborg Edén, C. Adhesion ofStreptococcus pneumoniae to human pharyngeal epithelial cells in vitro: differences in adhesive capacity among strains isolated from subjects with otitis media, septicemia, or meningitis or from healthy carriers. Infection and Immunity 1981, 32: 311–317.
Wallace, R.J., Baker, C.J., Quinones, F.J., Hollis, D.G., Weaver, R.E., Wiss, K. NontypableHaemophilus influenzae (biotype 4) as a neonatal, maternal, and genital pathogen. Reviews of Infectious Diseases 1983, 5: 123–136.
Controni, G., Chang, M.J., Gold, B.G., Rodriguez, W.J. Haemophilus influenzae biotype III infections in children and report of three unusual cases. American Journal of Clinical Pathology 1981, 76: 718–720.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dabernat, H., Delmas, C., Rich, C. et al. Characterization ofHaemophilus influenzae isolated from patients with otitis media. Eur. J. Clin. Microbiol. Infect. Dis. 7, 219–225 (1988). https://doi.org/10.1007/BF01963092
Issue Date:
DOI: https://doi.org/10.1007/BF01963092