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Commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence

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Abstract

A commercially available direct immunofluorescence (IF) test using a reagent consisting of a pool of two monoclonal antibodies against selected surface and internal proteins of respiratory syncytial virus (RSV) was evaluated in comparison with the indirect IF technique using a commercial bovine anti-RSV hyperimmune serum for the rapid detection of RSV in nasopharyngeal aspirates from 228 hospitalized children. Overall agreement between the two IF methods was 95%. The direct IF test was quicker to perform and easier to interpret than the indirect IF test.

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Authors and Affiliations

  1. Laboratory of Microbiology, Faculty of Medicine of Saint-Etienne, rue Ambroise Paré, 42023, Saint-Etienne Cedex, France

    B. Pozzetto, O. G. Gaudin, A. Ros & S. Tartavel-Mermet

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  1. B. Pozzetto
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  2. O. G. Gaudin
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  3. A. Ros
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  4. S. Tartavel-Mermet
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Pozzetto, B., Gaudin, O.G., Ros, A. et al. Commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence. Eur. J. Clin. Microbiol. Infect. Dis. 7, 201–203 (1988). https://doi.org/10.1007/BF01963083

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  • DOI: https://doi.org/10.1007/BF01963083

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