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Antibody response to pertussis toxin in patients with clinical pertussis measured by enzyme-linked immunosorbent assay

  • G. Zackrisson
  • I. Krantz
  • T. Lagergård
  • P. Larsson
  • R. Sekura
  • N. Sigurs
  • J. Taranger
  • B. Trollfors
Article

Abstract

Serum antibody response to pertussis toxin was measured by enzyme-linked immunosorbent assay in 172 patients with clinical symptoms typical of whooping cough. The diagnosis was verified by culture in 100 patients. Serum antibodies were either not detectable or present only at low levels in sera obtained in the early stage of disease. Significant changes in serum levels of IgG, IgM and/or IgA were demonstrated in 143 patients (83 %). The lack of comparable increases in most of the other patients may be due to inappropriate timing of serum collection. Thus, detection of antibodies against pertussis toxin in paired serum samples can be used for serological diagnosis of pertussis. However, the presence of IgM and/or IgA in a single serum sample does not confirm a diagnosis of pertussis, since such antibodies were found in healthy adults as well as in patients two years after the disease. High levels of these antibodies are, however, suggestive of on-going or recent disease.

Keywords

Serum Level Serum Sample Antibody Response Pertussis Healthy Adult 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pittman, M. The concept of pertussis as a toxin-mediated disease. Pediatric Infectious Disease 1985, 3: 467–486.Google Scholar
  2. 2.
    Oda, M., Cowell, J. L., Burstyn, D. G., Manclark, C. R. Protective activities of the filamentous hemagglutinin and the lymphocytosis-promoting factor ofBordetella pertussis in mice. Journal of Infectious Diseases 1984, 150: 823–833.Google Scholar
  3. 3.
    Kwantes, W., Joynson, D. H. M., Williams, W. O. Bordetella pertussis isolation in general practice: 1977–1979 whooping cough epidemic in West Glamorgan. Journal of Hygiene 1983, 90: 149–158.Google Scholar
  4. 4.
    Mertsola, J., Kuronen, T., Turunen, A., Viljanen, M. K., Ruuskanen, O. Diagnosis of pertussis. Journal of Infection 1984, 8: 149–156.Google Scholar
  5. 5.
    Granström, M., Granström, G., Gillenius, P., Askelöf, P. Neutralizing antibodies to pertussis toxin in whooping cough. Journal of Infectious Diseases 1985, 151: 646–649.Google Scholar
  6. 6.
    Burstyn, D. G., Baraff, L. O., Peppler, M. S., Leake, R. D., Geme, J. St., Manclark, C. R. Serological response to filamentous hemagglutinin and lymphocytosis-promoting toxin ofBordetella pertussis. Infection and Immunity 1983, 41: 1150–1156.Google Scholar
  7. 7.
    Zackrisson, G., Lagergård, T., Lönnroth, I. An enzymelinked immunosorbent assay method for detection of immunoglobulins to pertussis toxin. Acta Pathologica et Microbiologica Scandinavica (C) 1986, 94: 227–231.Google Scholar
  8. 8.
    Granström, M., Lindberg, A. A., Askelöf, P., Hederstedt, B. Detection of antibodies in human serum against the fimbrial haemagglutinin ofBordetella pertussis by enzyme-linked immunosorbent assay. Journal of Microbiology 1982, 15: 85–96.Google Scholar
  9. 9.
    Granström, M., Granström, G., Lindfors, A., Askelöf, P. Serologic diagnosis of whooping cough by an enzymelinked immunosorbent assay using fimbrial haemagglutinin as antigen. Journal of Infectious Diseases 1982, 146: 741–745.Google Scholar
  10. 10.
    Sekura, R. D., Fish, F., Manclark, C. R., Meade, B., Zhang, Y. Pertussis toxin. Affinity purification of a new ADP-ribosyltransferase. Journal of Biological Chemistry 1983, 258: 14647–14651.Google Scholar
  11. 11.
    Viljanen, M. K., Ruuskanen, O., Granberg, C., Salmi, T. T. Serological diagnosis of pertussis: IgM, IgA and IgG antibodies againstBordetella pertussis measured by enzyme-linked immunosorbent assay. Scandinavian Journal of Infectious Diseases 1982, 14: 117–122.Google Scholar
  12. 12.
    Nagel, J., Poot-Scholtens, E. J. Serum IgA antibody toBordetella pertussis as an indicator of infection. Journal of Medical Microbiology 1983, 16: 417–426.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • G. Zackrisson
    • 1
  • I. Krantz
    • 2
  • T. Lagergård
    • 3
  • P. Larsson
    • 1
  • R. Sekura
    • 4
  • N. Sigurs
    • 5
  • J. Taranger
    • 6
  • B. Trollfors
    • 7
  1. 1.Department of Clinical BacteriologyUniversity of GöteborgGöteborgSweden
  2. 2.Department of Infectious DiseasesUniversity of GöteborgGöteborgSweden
  3. 3.Institute of Medical MicrobiologyUniversity of GöteborgGöteborgSweden
  4. 4.National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA
  5. 5.Department of PediatricsBorås HospitalBoråsSweden
  6. 6.Outpatient Department of PediatricsVästra Frölunda HospitalVästra FrölundaSweden
  7. 7.Department of Infectious DiseasesUniversity of UmeåUmeåSweden

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