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Effects of low temperatures on survival of frozen-thawed mouse embryos

Experientia volume 35, pages 1505–1506 (1979)Cite this article

Summary

Almost no damage to mouse morulae was observed between 0 and −40°C, and freezing damage to embryos in DMSO, glycerol or ethylene glycol occurred after exposure to −60, −50 or −50°C, respectively. Cooling embryos in DMSO or glycerol to temperatures below −50 to −60°C increased freezing damage. To the contrary, in the presence of ethylene, glycol, no more damage occurred after exposure to temperatures below −50°C.

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References

  1. R. R. Maurer, H. Bank and R. E. Staples, Biol. Reprod.16, 139 (1977).

    Google Scholar 

  2. P. Mazur, in: Cryobiology, p. 213. Ed. H. T. Meryman, Academic Press, New York and London 1966.

    Google Scholar 

  3. S. P. Leibo, J. J. McGrath and E. G. Cravalho, Cryobiology15, 257 (1978).

    Google Scholar 

  4. T. A. Parkening, Y. Tsunoda and M. C. Chang, J. exp. Zool.197, 369 (1976).

    Google Scholar 

  5. D. G. Whittingham, S. P. Leibo and P. Mazur, Science178, 411 (1972).

    Google Scholar 

  6. D. G. Whittingham, Cryobiology15, 245 (1978).

    Google Scholar 

  7. K. Utsumi and M. Yuhara, Sci. Rep. Fac. Agr. Okayama Univ.44, 24 (1974).

    Google Scholar 

  8. S. M. Willadsen, C. Polge, L. E. A. Rowson and R. M. Moor, J. Reprod. Fert.46, 151 (1976).

    Google Scholar 

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Affiliations

  1. Department of Animal Science, College of Agriculture, Kyoto University, 606, Kyoto, Japan

    H. Miyamoto & T. Ishibashi

Authors
  1. H. Miyamoto
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  2. T. Ishibashi
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Miyamoto, H., Ishibashi, T. Effects of low temperatures on survival of frozen-thawed mouse embryos. Experientia 35, 1505–1506 (1979). https://doi.org/10.1007/BF01962812

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  • DOI: https://doi.org/10.1007/BF01962812

Keywords

  • Ethylene
  • Glycerol
  • DMSO
  • Glycol
  • Mouse Embryo