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Evaluation of modified trypticase soy broth versus supplemented peptone broth in the detection of bacteremia and fungemia

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Abstract

In vitro, 1.2% gelatin counteracts the inhibition of growth of bacterial species by sodium polyanetholsulfonate in blood culture media. Additionally, 1% yeast extract has been used to promote bacterial growth. We compared the performance of supplemented peptone broth and Trypticase soy broth, both of which contained sodium polyanetholsulfonate, gelatin and yeast extract. Trypticase soy broth with gelatin and yeast extract inhibited (p<0.001) and delayed growth, especially of gram-positive (p < 0.01) and gram-negative (p<0.005) anaerobic bacteria. Although the recovery of organisms usually inhibited by sodium poly-anetholsulfonate was similar in supplemented peptone and Trypticase soy broths, supplemented peptone broth clearly was superior in the recovery of other organisms commonly found in blood cultures.

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Authors and Affiliations

  1. Department of Pathology 13, Veterans Administration Medical Center, 84148, Salt Lake City, Utah, USA

    L. Reimer

  2. Department of Medicine, University of Colorado School of Medicine, 80262, Denver, Colorado, USA

    L. Reimer, B. Reller & S. Mirrett

  3. Department of Pathology, University of Colorado School of Medicine, 80262, Denver, Colorado, USA

    W. -L. Wang

Authors
  1. L. Reimer
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  2. B. Reller
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  3. W. -L. Wang
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  4. S. Mirrett
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Reimer, L., Reller, B., Wang, W.L. et al. Evaluation of modified trypticase soy broth versus supplemented peptone broth in the detection of bacteremia and fungemia. Eur. J. Clin. Microbiol. Infect. Dis. 7, 384–387 (1988). https://doi.org/10.1007/BF01962342

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