Abstract
Serum samples were obtained from 44 infants vaccinated against diphtheria at the ages of 3, 5 and 12 months with an aluminium-adsorbed diphtheria-tetanus toxoid vaccine containing 15 Lf units of diphtheria toxoid. Toxin-neutralizing antibodies (antitoxin) were measured by the Vero cell assay and IgG, IgM and IgA antibodies against diphtheria toxoid by enzyme-linked immunosorbent assay. A neutralizing antibody titer of 10 corresponded to 0.01 IU/ml, the level considered necessary for short-term protection. Geometric mean neutralizing antibody titers at 3, 5, 6, 12, 13 and 30 months were 28, 21, 173, 61, 1076 and 61. All children had titers of ≥ 10 (≥ 0.01 IU/ml) between 6 and 30 months of age. At 30 months only 48 % had titers of ≥ 100 (≥ 0.1 IU/ml), the level considered necessary for long-term protection. Geometric mean IgG antibody levels were 13, 36, 216, 64, 649 and 57. IgG antibodies significantly correlated with neutralizing titers and predicted neutralizing antibodies above or below 10 and 100 with an accuracy of 96 and 82 %, respectively. IgG antibodies could not, however, be used to predict individual neutralizing antibody titers with great accuracy. IgM antibodies were only detected after the third vaccination. IgA antibodies were not detected in any serum sample from ten infants tested. In conclusion, the Swedish vaccination schedule results in protective antibody levels in infants until at least 30 months of age. The decline of the antibody titers indicates a need for further studies to establish the duration of protection.
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Brooks GH, Bennet JV, Feldman R: Diphtheria in the United States, 1959–1970. Journal of Infectious Diseases 1971, 129: 172–178.
Björkholm B, Böttiger M, Christenson B, Hagberg L: Antitoxin antibody levels and the outcome of illness during an outbreak of diphtheria among alcoholics. Scandinavian Journal of Infectious Diseases 1986, 18: 235–239.
Claesson BA, Schneerson R, Robbins JB, Johansson J, Lagergård T, Taranger J, Bryla D, Levi L, Cramton T, Trollfors B: Protective levels of serum antibodies stimulated in infants by two injections ofHaemophilus influenzae type b capsular polysaccharide-tetanus toxoid conjugate. Journal of Pediatrics 1989, 114: 97–100.
Miyamura K, Nishio S, Ito A, Murata R, Kono R: Micro cell culture method for determination of diphtheria toxin and antitoxin titres using Vero cells. I. Studies on factors affecting the toxin and antitoxin titration. Journal of Biological Standardization 1974, 2: 189–201.
Edsall G: Immunization of adults against diphtheria and tetanus. American Journal of Public Health 1952, 42: 393–400.
Ipsen J: Circulating antitoxin at the onset of diphtheria in 425 patients. Journal of Immunology 1946, 54: 325–447.
Scheibel I, Tulinius S, Rasch G, Bojlen K, Borg Petersen C: Immunization of adults against diphtheria with particular reference to dosage and reactions. Acta Pathologica et Microbiologica Scandinavica 1948, 25: 319–350.
Orenstein OA, Weisfeld JS, Halsey NA: Diphtheria and tetanus toxoids and pertussis toxoid, combined. Publication no. 451. Pan American Health Organization WHO, Washington, 1983, p. 30–51.
Christensen PA, Pollak O, Carson S: The dose of diphtheria toxoid in combined vaccines and their use in children. South African Medical Journal 1980, 57: 689–692.
Simonsen O: Vaccination against tetanus and diphtheria. Danish Medical Bulletin 1989, 36: 24–47.
Christenson B, Böttiger M: Serological immunity to diphtheria in Sweden in 1978 and 1984. Scandinavian Journal of Infectious Diseases 1986, 18: 227–233.
Mark A, Christenson B, Granström M, Strandell A, Wickbom B, Böttiger M: Immunity and immunization of children against diphtheria in Sweden. European Journal of Clinical Microbiology and Infectious Diseases 1989, 8: 214–219.
Krantz I, Sekura R, Trollfors B, Taranger J, Zackrisson G, Lagergård T, Schneerson R, Robbins J: Immunogenicity and safety of a pertussis vaccine, composed of pertussis toxin inactivated by hydrogen peroxide, in 18-to 23-month-old children. Journal of Pediatrics 1990, 116: 539–543.
Camargo ME, Silveira L, Furuta JA, Oliveira EPT, Germek OA: Immunoenzymatic assay of anti-diphtheric toxin antibodies in human serum. Journal of Clinical Microbiology 1984, 20: 772–774.
Miyamura K, Tajiri E, Ito A, Murata R, Kono R: Micro cell culture method for determination of diphtheria toxin and antitoxin titres using Vero cells. II. Comparison with the rabbit skin method and practical application for seroepidemiological studies. Journal of Biological Standardization 1974, 2: 203–209.
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Lagergård, T., Trollfors, B., Claesson, B.A. et al. Determination of neutralizing antibodies and specific immunoglobulin isotype levels in infants after vaccination against diphtheria. Eur. J. Clin. Microbiol. Infect. Dis. 11, 341–345 (1992). https://doi.org/10.1007/BF01962074
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DOI: https://doi.org/10.1007/BF01962074