Skip to main content
SpringerLink
Log in

Evaluation of three rapid enzyme immunoassays and cell culture for detection of respiratory syncytial virus

  • Notes
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

Three rapid enzyme immunoassay techniques for the detection of respiratory syncytial virus antigen (Becton Dickinson Directigen RSV, Abbott RSV Testpack and Abbott RSV EIA) and cell culture were evaluated in a total of 250 nasal washings. The sensitivity and specificity were 62 % and 76 % respectively for Directigen, 64 % and 86 % for RSV Testpack, and 76 % and 81 % for RSV EIA, taking cell culture as the reference method. Agreement between cell culture and EIA techniques was 79 % (70 positive and 128 negative results). All three EIA techniques gave positive results in 69 samples (52 positive and 17 negative in the cell culture). In 121 samples all three EIA techniques gave negative results (103 negative and 18 positive in the cell culture). Using the cell culture technique 46 strains other than respiratory syncytial virus were isolated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Demers RR, Parker J, Frankel LR, Smith DW: Administration of ribavirin to neonatal and pediatric patients during mechanical ventilation. Respiratory Care 1986, 31: 1188–1196.

    PubMed  Google Scholar 

  2. MacDonald NE, Hall CB, Suffin SC, Alexson C, Harris PJ, Manning JA: Respiratory syncytial viral infection in infants with congenital heart diseases. New England Journal of Medicine 1982, 307: 397–400.

    PubMed  Google Scholar 

  3. Goldmann DA: Nosocomial viral infections: recent developments and new strategies. European Journal of Clinical Microbiology & Infectious Diseases 1989, 8: 75–81.

    Google Scholar 

  4. Halstead DC, Todd S, Fritch G: Evaluation of five methods for respiratory syncytial virus detection. Journal of Clinical Microbiology 1990, 28: 1021–1025.

    PubMed  Google Scholar 

  5. Kok T, Barancek K, Burrell CJ: Evaluation of Becton Dickinson Directigen test for respiratory syncytial virus in nasopharyngeal aspirates. Journal of Clinical Microbiology 1990, 28: 1458–1459.

    PubMed  Google Scholar 

  6. Swierkosz EM, Flanders R, Melvin L, Miller JD, Kline MW: Evaluation of the Abbott Testpack RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. Journal of Clinical Microbiology 1989, 27: 1151–1154.

    PubMed  Google Scholar 

  7. Wren CG, Bate BJ, Masters HB, Launer BA: Detection of respiratory syncytial virus antigen in nasal washings by Abbott Testpack enzyme immunoassay. Journal of Clinical Microbiology 1990, 28: 1395–1397.

    PubMed  Google Scholar 

  8. Rothbarth PH, Hermus MC, Schrijnemakers P: Reliability of two new test kits for rapid diagnosis of respiratory syncytial virus infection. Journal of Clinical Microbiology 1991, 29: 824–826.

    PubMed  Google Scholar 

  9. Grover S, Watkins P, Orvell C, Booth J: Comparison of direct immunofluorescence of exfoliated cells, tissue culture immunofluorescence and conventional virus isolation for the diagnosis of respiratory syncytial virus infections. Serodiagnosis and Immunotherapy in Infectious Diseases 1990, 4: 59–66.

    Article  Google Scholar 

  10. Johnston SLG, Siegel CS: Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. Journal of Clinical Microbiology 1990, 28: 2394–2397.

    PubMed  Google Scholar 

  11. Mendoza J, Navarro JM, Rojas A, de la Rosa M: Evaluation of immunofluorescence, two enzyme immunoassays and the shell-vial assay for detection of respiratory syncytial virus. European Journal of Clinical Microbiology & Infectious Diseases 1991, 10: 40–42.

    Google Scholar 

  12. Waner JL, Whitehurs NJ, Todd SJ, Shalaby H, Wall L: Comparison of Directigen RSV with viral isolation and direct immunofluorescence for identification of respiratory syncytial virus. Journal of Clinical Microbiology 1990, 28: 480–483.

    PubMed  Google Scholar 

  13. Thomas EE, Book LE: Comparison of two rapid methods for detection of respiratory syncytial virus (Testpack RSV and Ortho RSV ELISA) with direct immunofluorescence and virus isolation for the diagnosis of pediatric RSV infection. Journal of Clinical Microbiology 1991, 29: 632–635.

    PubMed  Google Scholar 

  14. Hornsleth A: A rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion. European Journal of Clinical Microbiology & Infectious Diseases 1990, 9: 356–358.

    Google Scholar 

  15. Frayha H, Castriciano S, Mahony J, Chernesky M: Nasopharyngeal swabs and nasopharyngeal aspirates are equally effective for the diagnosis of viral respiratory disease in hospitalized children. Journal of Clinical Microbiology 1989, 27: 1387–1389.

    PubMed  Google Scholar 

  16. Chonmaitree T, Bessette-Henderson BJ, Hepler R, Lucia H: Comparison of three rapid diagnostic techniques for detection of respiratory syncytial virus from wash specimens. Journal of Clinical Microbiology 1987, 25: 746–747.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Servicio de Microbiologia, Hospital Regional de Especialidades Virgen de las Nieves, E-19014, Granada, Spain

    J. Mendoza, A. Rojas, J. M. Navarro, C. Plata & M. de la Rosa

Authors
  1. J. Mendoza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Rojas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Plata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. de la Rosa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mendoza, J., Rojas, A., Navarro, J.M. et al. Evaluation of three rapid enzyme immunoassays and cell culture for detection of respiratory syncytial virus. Eur. J. Clin. Microbiol. Infect. Dis. 11, 452–454 (1992). https://doi.org/10.1007/BF01961862

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01961862

Keywords

Search

Navigation