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Serodiagnosis ofHelicobacter pylori infections with an enzyme immunoassay using the chromatographically purified 120 kilodalton protein

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Abstract

A membrane-associated 120 kDa protein ofHelicobacter pylori with known species-specificity was isolated and used in an enzyme immunoassay (EIA) for the detection ofHelicobacter pylori-specific IgG antibodies in patient sera. The EIA was compared with two other methods used for serodiagnosis ofHelicobacter pylori infections: an EIA using sonicated wholeHelicobacter pylori cell antigen and Western immunoblot. In a prospective study 127 unselected patients (76 patients with antrum gastritis, 51 patients without gastritis) who underwent gastroscopy were studied histologically and serologically. The EIA using the purified 120 kDa protein had the highest specificity (92 %) compared with the EIA using a whole cell sonicate of a singleHelicobacter pylori strain as antigen (60.7 %) and the immunoblot (90.2 %). The sensitivity was 96 %, 100 % and 92 %, respectively. Sera of three control patients reacted strongly in all three methods, indicating possibleHelicobacter pylori infection with negative histological findings. The EIA using the 120 kDa protein as antigen was shown to be a specific and sensitive technique for the serodiagnosis ofHelicobacter pylori infections.

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Authors and Affiliations

  1. Institute of Medical Microbiology, University of Freiburg, Hermann-Herder-Str. 11, 7800, Freiburg, Germany

    B. Gerstenecker, B. Eschweiler, H. Vögele & M. Kist

  2. Private Institute for Pathology, 7800, Freiburg, Germany

    H. K. Koch

  3. Institute of Pathology, University of Freiburg, Hermann-Herder-Str. 11, 7800, Freiburg, Germany

    U. Hellerich

Authors
  1. B. Gerstenecker
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  2. B. Eschweiler
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  3. H. Vögele
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  4. H. K. Koch
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  5. U. Hellerich
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  6. M. Kist
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Gerstenecker, B., Eschweiler, B., Vögele, H. et al. Serodiagnosis ofHelicobacter pylori infections with an enzyme immunoassay using the chromatographically purified 120 kilodalton protein. Eur. J. Clin. Microbiol. Infect. Dis. 11, 595–601 (1992). https://doi.org/10.1007/BF01961665

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