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Specific radioimmunoprecipitation of histone H2A antigens by protein A conjugated sepharose

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Summary

A modified radioimmunoprecipitation technique is described which allows the specific detection of histone H2A antigens. The technique circumvents unspecific binding of histones to the bacterial adsorbent.

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References

  1. Forsgren, A., and Sjöquist, J., J. Immun.97 (1966) 822.

    Article  CAS  PubMed  Google Scholar 

  2. Forsgren, A., and Sjöquist, J., J. Immun.99, (1967) 19.

    Article  CAS  PubMed  Google Scholar 

  3. Brunda, M. J., Minden, P., Sharpton, T.R., McClatchy, J. K., and Farr, R. S., J. Immun.119 (1977) 193.

    Article  CAS  PubMed  Google Scholar 

  4. Ivarie, R. D., and Jones, P. P., Analyt. Biochem.97, (1979) 24.

    Article  CAS  PubMed  Google Scholar 

  5. Platt, E. J., Karlsen, K., Lopez-Valdivieso, A., Cook, P. W., and Firestone, G. L., Analyt. Biochem.156 (1986) 126.

    Article  CAS  PubMed  Google Scholar 

  6. Ruder, F. J., Frasch, M., Mettenleiter, T. C., and Büsen, W., Devl Biol.122 (1987) 568.

    Article  CAS  Google Scholar 

  7. Frasch, M., Thesis, University of Tübingen, 1985.

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Authors and Affiliations

  1. Institut für Biologie II, Genetik, Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Germany

    F. J. Ruder & W. Büsen

  2. Department of Biological Sciences, Columbia University, 10027, New York, New York, USA

    M. Frasch

Authors
  1. F. J. Ruder
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  2. M. Frasch
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  3. W. Büsen
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Ruder, F.J., Frasch, M. & Büsen, W. Specific radioimmunoprecipitation of histone H2A antigens by protein A conjugated sepharose. Experientia 44, 347–348 (1988). https://doi.org/10.1007/BF01961278

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  • DOI: https://doi.org/10.1007/BF01961278

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