Skip to main content
SpringerLink
Log in

Use of genomic fingerprinting in the characterization ofNeisseria gonorrhoeae isolated in Valencia, Spain

  • Article
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

Ninety-fiveNeisseria gonorrhoeae organisms isolated in Valencia, Spain, were characterized by antibiotic sensitivity testing, auxotyping, serotyping, plasmid analysis and restriction endonuclease fingerprinting (HindIII digestion). Cluster analysis of the restriction patterns revealed that 31 isolates (32.6%) formed 12 clearly defined clusters. Penicillinase-producingNeisseria gonorrhoeae strains formed four of these groups. Eight groups of gonococcal strains were identified by auxotyping, although 83% of isolates belonged to two auxotypes (Proto, Pro-). Twenty-three different serovars were identified by serotyping. The serovar pattern IB/rop was found in 38% of isolates. A 60% coincidence was found between gonococcal groupings obtained by combination of auxotyping, serotyping and plasmid analysis and those obtained with the restriction enzyme fingerprinting technique. The specificity of enzyme restriction patterns ofNeisseria gonorrhoeae is confirmed to be of practical importance in the epidemiologic study of gonorrhoea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Falk ES, Bjorvatn B, Danielsson D, Kristiansen BE, Melby K, Sorensen H Restriction endonuclease fingerprinting of chromosomal DNA ofNeisseria gonorrhoeae. Acta Pathologica et Microbiologica Scandinavica (B) 1984, 92: 271–278.

    Google Scholar 

  2. Falk ES, Danielsson D, Bjorvatn B, Melby K, Sorensen B, Kristiansen BE Genomic fingerprinting in the epidemiology of gonorrhoea. Acta Dermato-Venereologica 1985, 65: 235–239.

    Google Scholar 

  3. Falk ES, Danielsson D, Bjorvatn H, Melby K, Sorensen B, Kristiansen BE, Lund S, Sandström E Phenotypic and genotypic characterization of penicillinase-producing strains ofNeisseria gonorrhoeae. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1985, 93: 91–97.

    Google Scholar 

  4. Falk E, Bjorvatn B, Kristiansen BE, Sorensen B, Melby K, Danielsson D Some phenotypic and genotypic characteristics of prevailing gonococcal strains in northern Norway. Genitourinary Medicine 1986, 62: 9–14.

    Google Scholar 

  5. Falk ES, Bygdeman SM, Birkeland NK, Bjorvatn B, Kallings I, Sandström EG Genotypes and phenotypes of β-lactamase producing strains ofNeisseria gonorrhoeae from African countries. Genitourinary Medicine 1988, 64: 226–232.

    Google Scholar 

  6. Falk ES, Egglestone ES, Digranes A, Volden G, Bjorvatn B Genotypic and phenotypic markers in the differentiation ofNeisseria gonorrhoeae strains. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1988, 96: 109–116.

    Google Scholar 

  7. Catlin BW Nutritional profiles ofNeisseria gonorrhoeae, Neisseria meningitidis, andNeisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. Journal of Infectious Diseases 1973, 128: 178–194.

    Google Scholar 

  8. Wang SP, Holmes KK, Knapp JS, Ott S, Kyzer DS Immunologic classification ofNeisseria gonorrhoeae with microimmunofluorescence. Journal of Immunology 1977, 119: 795–803.

    Google Scholar 

  9. Sandström E, Danielsson D Serology ofNeisseria gonorrhoeae. Classification by co-agglutination. Acta Pathologica et Microbiologica Scandinavica (B) 1980, 88: 27–38.

    Google Scholar 

  10. Tam MR, Buchanan TM, Sandström EG, Holmes KK, Knapp JS, Siadak AW, Nowinski RC Serological classification ofNeisseria gonorrhoeae with monoclonal antibodies. Infection and Immunity 1982, 36: 1042–1053.

    Google Scholar 

  11. Bygdeman S, Danielsson D, Sandström E Gonococcal W serogroups in Scandinavia. A study with polyclonal and monoclonal antibodies. Acta Pathologica et Microbiologica Scandinavica (B) 1983, 91: 293–305.

    Google Scholar 

  12. Sandström E, Lindell P, Harfast H, Blamberg F, Ryden AC, Bygdeman S Evaluation of a new set ofNeisseria gonorrhoeae serogroup W-specific monoclonal antibodies for serovar determination. In: Schoolnick GK (ed): The pathogenic Neisseriae. American Society for Microbiology, Washington, DC, 1985, p. 26–30.

    Google Scholar 

  13. Ericsson MM, Sherin JC Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathologica et Microbiologica Scandinavica (B) 1971, Supplement 217: 1–90.

    Google Scholar 

  14. Poh CL, Ocampo JC, Sng EH, Bygdeman SM Rapid in situ generation of DNA restriction endonuclease patterns forNeisseria gonorrhoeae. Journal of Clinical Microbiology 1989, 27: 2784–2788.

    Google Scholar 

  15. O'Callaghan CH, Morris A, Kirby SM, Shingler AH Novel method for detection of beta-lactamase by using a chromogenic cephalosporin substrate. Antimicrobial Agents and Chemotherapy 1972, 1: 283–288.

    Google Scholar 

  16. Birnboim HC, Doly J A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 1979, 7: 1513–1523.

    Google Scholar 

  17. Ish-Horowicz D, Burke JF Rapid and efficient cosmid cloning. Nucleic Acids Research 1981, 9: 2989–2998.

    Google Scholar 

  18. Macrina FL, Kopecko DJ, Jones KR, Ayers DJ, McCowen SM A multiple plasmid-containingEscherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978, 1: 417–420.

    Google Scholar 

  19. Bygdeman S, Killings I, Danielsson D Serogrouping and auxotyping for epidemiological study of β-lactamase producingNeisseria gonorrhoeae strains isolated in Sweden. Acta Dermato-Venereologica 1981, 6: 329–334.

    Google Scholar 

  20. Plikaitis BD, Carbone GM, Edmons P, Mayer LW Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base pair number after gel electrophoresis. Annals of Biochemistry and Experimental Medicine 1986, 152: 346–364.

    Google Scholar 

  21. Romsburg MC Cluster analysis for researchers. Lifetime Learning Publications, Belmont, CA, 1984.

    Google Scholar 

  22. Foster RS, Foster GC Electrophoretic comparison of endonuclease-digested plasmids fromNeisseria gonorrhoeae. Journal of Bacteriology 1976, 126: 1297–1304.

    Google Scholar 

  23. Mazloum H, Totten PA, Brooks GF, Dawson CR, Falkow S, James JF, Knapp JS, Koomey JM, Lammel CJ, Peters D, Schachter J, Tang WS, Vedros NA An unusualNeisseria isolated from conjunctival cultures in rural Egypt. Journal of Infectious Diseases 1986, 154: 212–225.

    Google Scholar 

  24. Roy RN, Bigelow N, Dillon JR A novel insertion sequence in the cryptic plasmid ofNeisseria gonorrhoeae may alter the B protein at the translational level. Plasmid 1988, 19: 39–45.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University Hospital, Faculty of Medicine, Avenida Blasco Ibánez 17, 46010, Valencia, Spain

    M. Dasi, J. M. Nogueira, J. J. Camarena, C. Gil, R. Garcia-Verdú, J. L. Barberá & J. Barberá

Authors
  1. M. Dasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. M. Nogueira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. J. Camarena
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Gil
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Garcia-Verdú
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. L. Barberá
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Barberá
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dasi, M., Nogueira, J.M., Camarena, J.J. et al. Use of genomic fingerprinting in the characterization ofNeisseria gonorrhoeae isolated in Valencia, Spain. Eur. J. Clin. Microbiol. Infect. Dis. 11, 804–809 (1992). https://doi.org/10.1007/BF01960879

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01960879

Keywords

Search

Navigation