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Evaluation of four commercial methods for identification and biotyping of genital and neonatal strains ofHaemophilus species

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Abstract

Four commercial methods for identification ofHaemophilus species were evaluated in comparison to conventional methods using 188 genital and neonatalHaemophilus strains. In the case of discrepancies between results obtained by the different methods, DNA-DNA hybridization was performed. The four commercial systems and conventional methods showed excellent correlation of results in 167 strains (88 %). DNA-DNA hybridization was performed in 8 strains with discrepant identification results and 13 strains with discrepant biotyping results. In 15 cases discrepancies could be explained by the fact that the strains belonged to a newly recognised species ofHaemophilus.

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References

  1. Doern GV, Chapin KC: Laboratory identification ofHaemophilus influenzae: effects of basal media on the results of satellitism test and evaluation of the RapID NH System. Journal of Clinical Microbiology 1984, 20: 599–601.

    PubMed  Google Scholar 

  2. Edberg SC, Melton E, Singer JM: Rapid biochemical characterization ofHaemophilus species by using the Micro ID. Journal of Clinical Microbiology 1980, 11: 22–26.

    PubMed  Google Scholar 

  3. Holmes RL, De Franco LM, Otto M: Novel method of biotypingHaemophilus influenzae that uses API 20E. Journal of Clinical Microbiology 1982, 15: 1150–1152.

    PubMed  Google Scholar 

  4. Janda WM, Bradna JJ, Ruther P: Identification ofNeisseria spp.,Haemophilus spp., and other fastidious gram-negative bacteria with the MicroScanHaemophilus-Neisseria identification panel. Journal of Clinical Microbiology 1989, 27: 869–873.

    PubMed  Google Scholar 

  5. Janda WM, Malloy PJ, Schreckenberger PC: Clinical evaluation of the VitekNeisseria-Haemophilus identification card. Journal of Clinical Microbiology 1987, 25: 37–41.

    PubMed  Google Scholar 

  6. Juni BA, Rysavy JM, Blazevic DJ: Rapid biotyping ofHaemophilus influenzae andHaemophilus para-influenzae with Patho Tec Strips and Spot Biochemical tests. Journal of Clinical Microbiology 1982, 15: 976–978.

    PubMed  Google Scholar 

  7. Matthews JS, Reynolds JA, Weesner DE, Perry JL, Jenkins AL: Rapid species identification and biotyping of respiratory isolates ofHaemophilus spp. Journal of Clinical Microbiology 1983, 18: 472–475.

    PubMed  Google Scholar 

  8. Palladino S, Leahy BJ, Newall TL: Comparison of the RIM-H rapid identification kit with conventional tests for the identification ofHaemophilus spp. Journal of Clinical Microbiology 1990, 28: 1862–1863.

    PubMed  Google Scholar 

  9. Quentin R, Musser JM, Mellouet M, Sizaret PY, Selander RK, Goudeau A: Typing of urogenital, maternal, and neonatal isolates ofHaemophilus influenzae andHaemophilus parainfluenzae in correlation with clinical source of isolation and evidence for a genital specificity ofH. influenzae biotype IV. Journal of Clinical Microbiology 1989, 27: 2286–2294.

    PubMed  Google Scholar 

  10. Quentin R, Goudeau A, Wallace RJ, Smith AL, Selander RK, Musser JM: Urogenital, maternal and neonatal isolates ofHaemophilus influenzae: identification of unusually virulent serologically nontypable clone families and evidence for a newHaemophilus species. Journal of General Microbiology 1990, 136: 1203–1209.

    PubMed  Google Scholar 

  11. Musser JM, Barenkamp SJ, Granoff DM, Selander RK: Genetic relationships of serologically nontypable and serotype b strains ofHaemophilus influenzae. Infection and Immunity 1986, 52: 183–191.

    PubMed  Google Scholar 

  12. Wallace RJ, Baker CJ, Quinones FJ, Hollis DG, Weaver RE, Wiss K: NontypableHaemophilus influenzae (biotype 4) as a neonatal, maternal, and genital pathogen. Reviews of Infectious Diseases 1983, 5: 123–136.

    PubMed  Google Scholar 

  13. Kilian M:Haemophilus. In: Lennette EH, Balows A, Hausler WJ, Shadomy HJ (ed): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1985, p. 387–393.

    Google Scholar 

  14. Kilian M, Sorensen I, Fredericksen W: Biochemical characteristics of 130 recent isolates fromHaemophilus influenzae meningitis. Journal of Clinical Microbiology 1979, 9: 409–412.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Département de Microbiologie Médicale et Moléculaire, URA CNRS 1334, Centre Hospitalier Universitaire Bretonneau, 37044, Tours Cedex, France

    R. Quentin, I. Dubarry, C. Martin, B. Cattier & A. Goudeau

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  1. R. Quentin
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  2. I. Dubarry
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  3. C. Martin
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  4. B. Cattier
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  5. A. Goudeau
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Quentin, R., Dubarry, I., Martin, C. et al. Evaluation of four commercial methods for identification and biotyping of genital and neonatal strains ofHaemophilus species. Eur. J. Clin. Microbiol. Infect. Dis. 11, 546–549 (1992). https://doi.org/10.1007/BF01960812

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  • DOI: https://doi.org/10.1007/BF01960812

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