Experientia

, Volume 35, Issue 5, pp 707–707 | Cite as

A source of error in the fluorometric determination of protein in human placental microsomes

  • C. H. Blomquist
  • C. E. Kotts
Specialia Pro Experimentis

Summary

When protein is assayed in human placental microsomes by the fluorescamine procedure, 25–30% of the observed fluorescence results from labeling of phospholipids.

Keywords

Fluorescamine Fluorescence Result Fluorometric Determination Placental Microsome Human Placental Microsome 

References

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    P.V. Johnstone, Basic Lipid Methodology, p.26. Special Publication No. 19, College of Agriculture, University of Illinois, Urbana, Illinois, USA 1971.Google Scholar
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    The authors are grateful to Dr James Bryant Howard, University of Minnesota, for these analyses.Google Scholar
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Copyright information

© Birkhäuser Verlag 1979

Authors and Affiliations

  • C. H. Blomquist
    • 1
    • 2
  • C. E. Kotts
    • 1
    • 2
  1. 1.Department of Obstetrics and GynecologySt. Paul-Ramsey HospitalSt. PaulUSA
  2. 2.the University of Minnesota Medical SchoolMinneapolisUSA

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