, Volume 35, Issue 5, pp 666–668 | Cite as

Promotion of epithelial keratinization by N-methyl-N′-nitro-N-nitrosoguanidine in rat forestomach in organ culture

  • H. Fukamachi
  • S. Takayama


The effect of N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) on epithelial differentiation of fetal rat forestomach was investigated in organ culture. When forestomach tissues removed from 16.5-day fetuses were treated with 5 μg and 3 μg of MNNG per ml for 1 h, epithelial keratinization was observed after 4 and 5 days, respectively, whereas it occurred after 6 days in control cultures. A clear dose-response relationship was found in the promotion of epithelial keratinization by MNNG.


Organ Culture Control Culture Epithelial Differentiation Epithelial Keratinization 
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Copyright information

© Birkhäuser Verlag 1979

Authors and Affiliations

  • H. Fukamachi
    • 1
    • 2
  • S. Takayama
    • 1
    • 2
  1. 1.Zoological Institute, Faculty of ScienceUniversity of TokyoTokyo
  2. 2.Department of Experimental PathologyCancer InstituteTokyo(Japan)

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