, Volume 35, Issue 5, pp 660–662 | Cite as

Murine primitive neuroectodermal tumor: nucleolar isolation and RNA polymerase inhibition

  • D. E. Slagel
  • W. Kauffman


A nucleolar isolation procedure was developed for the murine primitive neuroectodermal tumor originally induced with methylcholanthrene. This procedure utilizes sonication for breakage of the nuclei at a magnesium concentration which disperses the extranucleolar chromatin. The nucleoli retain RNA synthetic activity which is inhibited to about 90% by actinomycin D and the chemotherapeutic drug adriamycin.


Magnesium Adriamycin Chemotherapeutic Drug Actinomycin Isolation Procedure 
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Copyright information

© Birkhäuser Verlag 1979

Authors and Affiliations

  • D. E. Slagel
    • 1
  • W. Kauffman
    • 1
  1. 1.Laboratory for Neurochemistry, Division of Neurosurgery, Department of SurgeryUniversity of Kentucky College of MedicineLexingtonUSA

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