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Shoot regeneration fromAgrobacterium tumefaciens-induced tumors of a tropical timber tree,Fagraea fragrans

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Agrobacterium tumefaciens A208 with nopaline plasmid pTiT37 was used to obtain stem tumors on plantlets ofFagraea fragrans grown in vitro. Bacterial elimination and tissue proliferation were simultaneously achieved by growing tumors on cefatoxime medium. After some tissue growth the shoots regenerated. An examination of these showed the presence of nopaline, indicating genetic transformation by T-DNA.

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References

  1. Acknowledgments. We thank Dr H. Kamada and Dr T. Nagata for valuable assistance and useful discussion. This work was supported by two research grants, one from the National University of Singapore (RP 62/83) and the other from Plantek International (Pte.) Ltd. (GR05348N).

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Authors and Affiliations

  1. Botany Department, National University of Singapore, Lower Kent Ridge Road, 0511, Singapore, Singapore

    C. S. Lon & A. N. Rao

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  1. C. S. Lon
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  2. A. N. Rao
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Lon, C.S., Rao, A.N. Shoot regeneration fromAgrobacterium tumefaciens-induced tumors of a tropical timber tree,Fagraea fragrans. Experientia 44, 72–73 (1988). https://doi.org/10.1007/BF01960253

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  • DOI: https://doi.org/10.1007/BF01960253

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