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Effect of amniotic fluid and fetal bovine serum on the morphogenesis of mouse duodenal villi in organ culture

Experientia volume 37, pages 417–418 (1981)Cite this article

Summary

When duodenal explants of 15-day mouse foetuses are cultured in Trowell T8 medium during 72 h, villi do not develop. If mouse amniotic fluid (25%) is added to the same medium, short villi appear after 12 h of culture and medium size villi are seen at 48 h. Bovine amniotic fluid and fetal bovine serum promote cell growth but morphogenesis of intestinal villi is far less stimulated.

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Affiliations

  1. Intestinal Biology Unit, Department of Anatomy and Cell Biology, University of Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    R. Calvert

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  1. R. Calvert
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Additional information

Supported by grand MA-6069 from the Medical Research Council of Canada.

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Calvert, R. Effect of amniotic fluid and fetal bovine serum on the morphogenesis of mouse duodenal villi in organ culture. Experientia 37, 417–418 (1981). https://doi.org/10.1007/BF01959896

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  • DOI: https://doi.org/10.1007/BF01959896

Keywords

  • Cell Growth
  • Bovine Serum
  • Fetal Bovine Serum
  • Organ Culture
  • Amniotic Fluid