, Volume 38, Issue 10, pp 1208–1210 | Cite as

Significance of nitrate reductase in nitrate assimilation in free-livingRhizobium cultures

  • M. H. Siddiqui
  • Anjali Mathur
  • S. N. Mathur
Short Communications Biochemistry


An in situ method for measuring nitrate reductase (NR) in free-livingRhizobium cultures has been developed. Arginine, threonine, valine, glutamic acid, tyrosine and tryptophan were found to promote NR synthesis. In root nodules rhizobial nitrate reductase also converts nitrate into NH4+, thus it provides an alternative to the nitrogenase route for the formation of NH4+.


Nodule Nitrate Tyrosine Arginine Assimilation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 3.
    E. Itagaki, T. Fujita and R. Sato, J. Biochem.53, 389 (1963).Google Scholar
  2. 4.
    L. Beevers and R.H. Hageman, A. Rev. Pl. Physiol.20, 495 (1969).Google Scholar
  3. 5.
    R.M. Daniel and C.A. Appleby, Biochim. biophys. Acta275, 347 (1972).Google Scholar
  4. 6.
    J.R. Manhart and P.P. Wong, Pl. Physiol. suppl.61, 3 (1978).Google Scholar
  5. 7.
    R.A. Ludwig, J. Bact.135, 114 (1978).Google Scholar
  6. 8.
    R.W. Jackson and J.A. DeMoss, J. Bact.90, 1420 (1965).Google Scholar
  7. 9.
    V.P. Choudary and G.R. Rao, Can. J. Microbiol.22, 35 (1976).Google Scholar
  8. 10.
    E.K. Allen and O.N. Allen, Bact. Rev.14, 273 (1950).Google Scholar
  9. 11.
    R.H. Hageman and D.P. Hucklesby, in: Methods in enzymology, vol. 23, p. 491. Ed. A. San Pietro. Academic Press, London 1971.Google Scholar
  10. 12.
    E.W. Yemm and E.C. Cocking, Analyst80, 209 (1955).Google Scholar
  11. 13.
    C.M. Johnson and A. Ulrich, Analyt. Chem.22, 1526 (1950).Google Scholar
  12. 14.
    L.D. Doneen, Pl. Physiol.7, 717 (1932).Google Scholar
  13. 15.
    D. Mukerji and I. Morris, Z. Pflanzenphysiol.90, 95 (1978).Google Scholar
  14. 16.
    R.C. Srivastava, D. Mukerji and S.N. Mathur, Ann. appl. Biol.96, 235 (1980).Google Scholar
  15. 17.
    J.W. Radin, Pl. Physiol.55, 178 (1975).Google Scholar
  16. 18.
    D. Rhodes and G.r. Stewart, Planta18, 133 (1974).Google Scholar
  17. 19.
    I.P. Solomonson and A.M. Spehar, Nature265, 373 (1977).Google Scholar
  18. 20.
    M. Aslam and A. Oaks, Pl. Physiol.57, 572 (1976).Google Scholar
  19. 21.
    M.G. Guerrero, J.M. Vega and M. Losada, A. Rev. Pl. Physiol.32, 169 (1981).Google Scholar

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • M. H. Siddiqui
    • 1
  • Anjali Mathur
    • 1
  • S. N. Mathur
    • 1
  1. 1.Plant Physiology Research Laboratory, Department of BotanyUniversity of GorakhpurGorakhpur(India)

Personalised recommendations