, Volume 38, Issue 10, pp 1181–1184 | Cite as

Analysis of nucleotides bound to the ATP synthetase from spinach chloroplasts isolated under different conditions

  • Stefan Bodmer
  • Reinhard Bachofen
Full Papers


The total amount of bound adenine nucleotides in the coupling factor isolated from spinach chloroplasts and its distribution on AMP, ADP and ATP was analyzed after various incubation conditions. During purification of the coupling factor, the distribution of AMP, ADP and ATP is not altered. The coupling factor from deenergized membranes contains approximately 1 ADP, less than 1 ATP, and small amounts of AMP. During phosphorylation the pattern is changed and ATP becomes the dominant species. When exogenous ADP is lacking, phosphate is readily incorporated into ATP. Inhibition of adenylate kinase by AP5A does not change the distribution pattern of the adenine nucleotides. The distribution pattern shows no integer numbers for the different nucleotides, suggesting that the coupling factor is present in different states in a statistical distribution.


Phosphate Nucleotide Purification Distribution Pattern Adenine 
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Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • Stefan Bodmer
    • 1
  • Reinhard Bachofen
    • 1
  1. 1.Institute of Plant Biology, MicrobiologyUniversity of ZürichZürich(Switzerland)

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