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In vitro translation of human placental pregnancy-specific beta1-glycoprotein (SP1)

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Summary

Synthesis of SP1-glycoprotein by the human placenta was directly demonstrated, by in vitro translation of RNA extracted from full term and from early placentas in a cell-free wheat germ system followed by specific immunoprecipitation of the radioactively labeled nascent peptides. De novo synthesized SP1-glycoprotein in both RNA preparations accounted for 1–1.3% of total protein synthesis.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, 10400, Bangkok, Thailand

    W. Hirunyavasit & T. Kusamran

Authors
  1. W. Hirunyavasit
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  2. T. Kusamran
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Acknowledgment. The authors thank Drs A. Nirapatpongporn, V. Sirivasin and Professor H.F. Lodish for kindly providing placental tissues and wheat germ respectively. This work was supported by The Rockefeller Foundation (RF-8031).

W.H. was supported by a Graduate Studies Fellowship, Mahidol University.

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Hirunyavasit, W., Kusamran, T. In vitro translation of human placental pregnancy-specific beta1-glycoprotein (SP1). Experientia 39, 206–207 (1983). https://doi.org/10.1007/BF01958903

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