Summary
One difficulty in the production of allophenic mice by aggregation of preimplantation embryos is that they frequently roll apart before the bonds between the blastomeres have had time to form. One solution to the problem, described here, is to pretreat one of the embryos with rabbit anti-mouse serum just prior to pushing them together. Blastocyst formation is unhampered by antibody treatment, and numerous allophenic mice have already been produced with this new procedure.
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References
Mintz, B., in Methods in mammalian embryology, p. 186, Ed. J.C. Daniel, Freeman, San Francisco 1971.
Mintz, B., A. Rev. Genet.8 (1974) 411.
Mintz, B., Science138 (1962) 594.
Levy, H.B., and Sober, H.A., Proc. Soc. exp. Biol. Med.103 (1960) 250.
Hall, B.V., Proc. Soc. exp. Biol. Med.32 (1935) 747.
Rossant, J., and Vikj, K.M., Devl Biol.76 (1980) 475.
Boyse, E.A., Hubbard, L., and Stockert, E., Transplantation10 (1970) 446.
Mintz, B., Gearhart, J.D., and Guymont, A.D., Devl Biol.31 (1973) 195.
Stanley, P., Caillibot, V., and Siminovitch, L., Somat. Cell Genet.1 (1975) 3.
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Acknowledgments. This investigation was supported in part by the Grace B. Reagin Memorial Grant for Cancer Research from the American Cancer Society and by a grant No. IN-107E, also from the American Cancer Society.
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Palmer, J., Dewey, M.J. Allophenic mice produced from embryos aggregated with antibody. Experientia 39, 196–198 (1983). https://doi.org/10.1007/BF01958897
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DOI: https://doi.org/10.1007/BF01958897