, Volume 39, Issue 2, pp 196–198 | Cite as

Allophenic mice produced from embryos aggregated with antibody

  • J. Palmer
  • M. J. Dewey


One difficulty in the production of allophenic mice by aggregation of preimplantation embryos is that they frequently roll apart before the bonds between the blastomeres have had time to form. One solution to the problem, described here, is to pretreat one of the embryos with rabbit anti-mouse serum just prior to pushing them together. Blastocyst formation is unhampered by antibody treatment, and numerous allophenic mice have already been produced with this new procedure.


Antibody Treatment Preimplantation Embryo Blastocyst Formation Allophenic Mouse 
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  1. 3.
    Mintz, B., in Methods in mammalian embryology, p. 186, Ed. J.C. Daniel, Freeman, San Francisco 1971.Google Scholar
  2. 4.
    Mintz, B., A. Rev. Genet.8 (1974) 411.CrossRefGoogle Scholar
  3. 5.
    Mintz, B., Science138 (1962) 594.Google Scholar
  4. 6.
    Levy, H.B., and Sober, H.A., Proc. Soc. exp. Biol. Med.103 (1960) 250.PubMedGoogle Scholar
  5. 7.
    Hall, B.V., Proc. Soc. exp. Biol. Med.32 (1935) 747.Google Scholar
  6. 8.
    Rossant, J., and Vikj, K.M., Devl Biol.76 (1980) 475.CrossRefGoogle Scholar
  7. 9.
    Boyse, E.A., Hubbard, L., and Stockert, E., Transplantation10 (1970) 446.PubMedGoogle Scholar
  8. 10.
    Mintz, B., Gearhart, J.D., and Guymont, A.D., Devl Biol.31 (1973) 195.CrossRefGoogle Scholar
  9. 11.
    Stanley, P., Caillibot, V., and Siminovitch, L., Somat. Cell Genet.1 (1975) 3.CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • J. Palmer
    • 1
  • M. J. Dewey
    • 1
  1. 1.Department of BiologyUniversity of South CarolinaColumbiaUSA

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