Summary
Sea urchin embryonic cells were made permeable by treating them with glycerol solution for the purpose of exchanging cytoplasmic components. When proteoglycans prepared from embryos of advanced stages of development permeated into these glycerinated cells, these substances accumulated rapidly in the nucleus, being bound with chromatin.
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This work was supported by grants-in-aid for cancer research from the Ministry of Education, Science and Culture, Japan.
Acknowledgment. The authors wish to express their gratitude to Dr T.E. Schroeder and Dr V. Meyer-Rochow for critical reading of the manuscript.
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Kinoshita, S., Yoshii, Y. Permeable embryonic cells of sea urchins as a model for studying nucleus-cytoplasm interactions. Experientia 39, 189–190 (1983). https://doi.org/10.1007/BF01958893
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DOI: https://doi.org/10.1007/BF01958893