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Monoclonal antibodies to metallothionein from Cd2+-resistant Chinese hamster lung fibroblasts

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Summary

Four monoclonal antibodies of the mouse against metallothioneins (MTs) from Cd2+-resistant fibroblasts of the Chinese hamster lung (Cdr-CHL) have been prepared. Each one of the antibodies showed a unique cross-reactivity pattern when tested against MTs from the livers of several mammals and from yeast.

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References

  1. Nordberg, M., and Kojima, Y., in: Metallothionein, p. 41. Eds J. H. R. Kägi and M. Nordberg, Birkhäuser, Basel 1979.

    Chapter  Google Scholar 

  2. Madapallimatam, G., and Riordam, J. R., Biochem. biophys. Res. Commun.77 (1977) 1286.

    Article  CAS  Google Scholar 

  3. Vander Mallie, R. J., and Garvey, J. S., Immunochemistry15 (1978) 857.

    Article  CAS  PubMed  Google Scholar 

  4. Vander Mallie, R. J., and Garvey, J. S., J. biol. Chem.254 (1979) 8416.

    Article  Google Scholar 

  5. Tohyama, C., and Shaikh, Z. A., Biochem. biophys. Res. Commun.84 (1978) 907.

    Article  CAS  PubMed  Google Scholar 

  6. Brady, F. O., and Kafka, R. L., Analyt. Biochem.98 (1979) 89.

    Article  CAS  PubMed  Google Scholar 

  7. Chang, C. C., Vander Mallie, R. J., and Garvey, J. S., Toxic. appl. Pharmac.55 (1980) 94.

    Article  CAS  Google Scholar 

  8. Köhler, G., and Milstein, C., Nature256 (1975) 495.

    Article  PubMed  Google Scholar 

  9. Oi, V. T., and Herzenberg, L. A., in: Selected methods in cellular immunology, p. 351. Eds B. B. Mishell and S. M. Shiigi. Freeman, San Francisco 1980.

    Google Scholar 

  10. Stocker, J. W., Malavasi, F., and Trucco, M., in: Immunological methods vol. 2, p. 299. Eds I. Lafkovits and B. Pernis. Academic Press, New York 1981.

    Chapter  Google Scholar 

  11. Köhler, G., in: Immunological methods, vol. 2, p. 296. Eds I. Lefkovits and B. Pernis. Academic Press, New York 1981.

    Google Scholar 

  12. Tsunoo, H., Kino, K., Nakajima, H., Hata, A., Huang, I-Y., and Yoshida, A., J. biol. Chem.253 (1978) 4172.

    Article  CAS  Google Scholar 

  13. Kimura, M., Otaki, N., and Imano, M., in: Metallothionein, p. 163. Eds J. H. R. Kägi and M. Nordberg. Birkhäuser, Basel 1979.

    Chapter  Google Scholar 

  14. Kimura, M., Otaki, N., and Kakefuda, T., in: Metallothionein, p. 187. Eds J. H. R. Kägi and M. Nordberg. Birkhäuser, Basel 1979.

    Chapter  Google Scholar 

  15. Weser, U., Hartmann, H-J., Fretzdorff, A., and Strobel, G-J., Biochim. biophys. Acta493 (1977) 465.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, Cancer Institute, Toshima-ku, 170, Tokyo, Japan

    T. Masui, T. Utakoji & M. Kimura

  2. Department of Experimental Toxicology, National Institute of Industrial Health, Tama-ku, 213, Kawasaki, Japan

    T. Masui, T. Utakoji & M. Kimura

Authors
  1. T. Masui
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  2. T. Utakoji
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  3. M. Kimura
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Additional information

This work was supported in part by a Grant-in-Aid for Cancer Research, the Ministry of Education, Science and Culture. We wish to thank to Dr T. Akatsuka, Dept. Genetics, Institute of Medical Science, University of Tokyo, for advice and for providing NS-1 cells, and Dr S. Tsukagoshi, Cancer Chemotherapy Center, for providing protein isolation facilities. The skillful technical assistance of Mrs K. Nemoto and Mrs N. Otaki is gratefully acknowledged.

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Masui, T., Utakoji, T. & Kimura, M. Monoclonal antibodies to metallothionein from Cd2+-resistant Chinese hamster lung fibroblasts. Experientia 39, 182–184 (1983). https://doi.org/10.1007/BF01958889

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